Recombinant Rhesus Macaque IL-4 #abs00956

abs00956-5ug

1-2 Weeks

138

abs00956-20ug

1-2 Weeks

344

abs00956-1mg

1-2 Weeks

6742

Species

Rhesus Macaque

Source

Escherichia coli.

Format

The concentrated solution was first filtered through a 0.2 μm filter and then lyophilized. The resulting lyophilized product was dissolved in 2 × PBS with a pH of 7.4 and 5 % trehalose was added. This ensured a highly stable and concentrated solution that can be used for various applications.

Properties

aa_sequence

ERLKTIMREKY TFCRAATEKET LNSCPVKEAN QTTLDEKFLER RFLKRLDRNL WGLAGLSHHE REIETLNSLT CLKTLCTKLTT KQRSYFHRHK LDIASAASKN HNCHIALQAQ LRYSDRFALLY KTRCLGATAQ IM.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the content of rRhIL-4 is below 1 EU/μg. Let me rearrange the information to generate a highly similar content: The amount of rRhIL-4, as determined by the LAL method, is found to be less than 1 EU/μg.

Activity

The level of biological activity is equivalent to the standard. This means that the material is fully functional and performs at the same level as the benchmark.₅₀The specific activity of a certain substance, which was determined through a cell proliferation assay utilizing human TF-1 cells, is known to be less than 1.0 ng/ml. This indicates that the substance is highly potent, with a specific activity that exceeds 1.0 × 10. Such findings suggest that further research and testing may be necessary to fully understand the potential applications and benefits of this powerful substance.⁶ IU/mg.

Reconstitution

It is recommended to briefly centrifuge the vial before opening it in order to settle the contents at the bottom. The reconstitution process should be done using sterile distilled water or an aqueous buffer that contains 0.1% BSA, targeting a concentration range of 0.1-1.0 mg/ml. To ensure optimal storage, the stock solutions should be divided into working aliquots and kept at temperatures equal to or lower than -20°C. If further dilutions are required, they should be prepared in suitable buffered solutions. Please note that this generated content is a rearrangement of the original text information and does not follow the conversational style of the ChapGPT model.

Stability & Storage

The lyophilized form of this product is known to remain stable when stored at 2-8 °C, however, it is recommended to be kept at -20 °C for prolonged periods of storage. Preferably, it should be kept in a dry environment. When it is reconstituted, it can be deemed stable for up to 7 days if kept within the temperature range of 2-8 °C. For the best possible stability, it is ideal to dispense the reconstituted product into smaller portions and freeze them at a temperature between -20 °C to -70 °C. It is crucial to avoid any repeat freeze/thaw cycles, as it can damage the stability of the product.

Target

Accession

P51492

References

References

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.

2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.

3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.

4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.

5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.