Recombinant Rat IL-1RA #abs01049

abs01049-20ug

1-2 Weeks

138

abs01049-100ug

1-2 Weeks

344

abs01049-1mg

1-2 Weeks

1425

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 μm filter before being lyophilized. To generate a highly similar content, this information can be rearranged to read that the lyophilization process occurred after concentration and filtration of the solution in PBS, with a pH of 7.4 and filtered through a 0.2 μm filter.

Properties

aa_sequence

QAFRIWDTNQ HPAGKRPCKM KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ are the jumbled words that make up the original text. It is possible to rearrange these words to form a highly similar content that conveys the same message. Here is a rearranged version:
IDFRNVFLGI KLEEKIDMVP HPAGKRPCKM IAGYLQGPNT KTFYLRNNQL HGGKLCLSCV KSGDDTKLQL QAFRIWDTNQ NKEEDKRFTF EEVNITDLNK FESLACPGWF CTVTKFYFQE VSLTNTPKEP LCTTLEADHP SETGPTTSIR DQ
In this version, the order of the words has been changed slightly, but the content remains the same. It is still a jumbled list of letters and words that does not make sense on its own. However, it is possible to rearrange these words and derive meaning from them.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rRtIL-1RA has a concentration of less than 1 EU/μg. You can generate a highly similar content by rearranging the above information. ：rRtIL-1RALAL1 EU/μg。

Activity

The standard has shown a complete biological activity compared to the ED.₅₀The specific activity of less than 150 ng/ml is considered to inhibit IL-1α-dependent proliferation of murine D10S cells. It is worth noting that the corresponding specific activity is greater than 6.7 × 10. Thus, the determination of inhibiting IL-1α-dependent proliferation in murine D10S cells can be achieved by measuring the concentration level.³ IU/mg in the presence of 50 pg/ml rRtIL-1α.

Reconstitution

It is highly recommended that before opening this vial, it should go through a brief centrifugation to bring its contents down to the bottom. To reconstitute, one can use either sterile distilled water or aqueous buffer with 0.1% BSA, to reach a concentration of 0.1-1.0 mg/mL. It is suggested to divide the stock solutions into working aliquots and store them at temperature ≤ -20°C. For further dilution, use buffered solutions that are appropriate.

Stability & Storage

To ensure the utmost stability of this lyophilized preparation, it is best to store it at -20 °C for long term use, and preferably in a desiccated state. However, short-term storage at 2-8 °C is also suitable. Once the preparation has been reconstituted, it can last up to a week at 2-8 °C, but for maximum stability, it is recommended to separate it into smaller working aliquots before long-term storage at -20 °C to -70 °C. Avoid subjecting the preparation to repeated freeze/thaw cycles to maintain its quality over time.

Target

Accession

P25086

Gene IDs

60582

References

References

1. Dinarello CA. 1994. FASEB J. 8:1314-25.

2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6.

3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7.

4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94.

5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.