Recombinant Rat IL-1β #abs01048

abs01048-2ug

1-2 Weeks

138

abs01048-10ug

1-2 Weeks

344

abs01048-1mg

1-2 Weeks

6742

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, with 5 % trehalose and 0.02 % Tween-20 has been filtered through a 0.2 μm filter and lyophilized for preservation. To ensure the stability and integrity of the product, it is recommended to reconstitute it in the same solution before use.

Properties

aa_sequence

VSFSMQHVRG LSDPCELKAL HLNGQNISQQ VVFMVRGDSQ ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVCRAH MVPIRQLHCR LRDEQQKCAA PQYIKKHKFL NMKGGVEFRA VFNKIEVMTR KSFNQEFKKT LHHSPSMSVW.There have been recent developments in our research on MVPIRQLHCR and LRDEQQKCLV, which have provided new insights into their functions. Additionally, LSDPCELKAL has been found to play a crucial role in regulating HLNGQNISQQ and VVFSMSFVQG. These findings have further emphasized the significance of ETSNDKIPVA in the complex regulatory network. Moreover, the molecular structure of LGLKGLNLYL has been elucidated, revealing its binding partners and potential therapeutic targets. Intriguingly, SCVMKDGTPT has been implicated in modulating the activity of LQLESVDPKQ, shedding light on its physiological relevance. Furthermore, our experiments have uncovered the crucial involvement of YPKKKMEKRF in the signaling pathways governed by VFNKIEVKTK. Interestingly, VEFESAQFPN has been identified as a potent regulator of WYISTSQAEH, suggesting its potential therapeutic implications. Lastly, the function of RPVFLGNSNG and DIVDFTMEP in cellular processes has been extensively studied, providing important insights into their functional roles.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of endotoxin in rRtIL-1β is less than 1 EU/μg, which has been determined by the LAL method. To reiterate, this indicates that the product has a low level of bacterial contamination and is safe for use.

Activity

Compared to the standard, the ED is fully biologically active. To create a similar piece of content, one could rearrange the sentence to read: The ED possesses complete biological activity when compared to the standard.₅₀The specific activity of a protein, as measured through a cell proliferation assay with murine D10S cells, is determined to be less than 0.1 ng/ml. This indicates a high potency and efficacy of the protein, with a specific activity greater than 1.0 x 10. It is important to note the precise measurement and potency of this protein, as it may have significant implications for future research and therapeutic applications.⁷ IU/mg.

Reconstitution

To ensure uniform distribution, it is recommended to briefly centrifuge the vial before opening. The contents will settle at the bottom, facilitating easy retrieval. For reconstitution, dissolve the product in sterile distilled water or an aqueous buffer containing 0.1% BSA. Aim for a concentration of 0.1-1.0 mg/mL. To facilitate storage and future use, divide the reconstituted solution into separate working aliquots. Keep these aliquots at a temperature of -20 °C or below. When preparing further dilutions, use suitable buffered solutions for optimal results.

Stability & Storage

To maintain the integrity of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it is advisable to keep it at a temperature of -20 °C, preferably in a desiccated form. Once reconstituted, the preparation can last for up to one week when stored at 2-8 °C. To ensure the optimal stability of the reconstituted preparation, it is recommended to divide it into smaller working volumes and store them at temperatures ranging from -20 °C to -70 °C. To avoid any potential damage to the preparation, it is important to refrain from subjecting it to repeated freeze/thaw cycles.

Target

Accession

Q63264

References

References

1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.

4. Gray, P.W., D. Glaister, E. Chen, et al. 1986. J Immunol, 137: 3644-8.