Recombinant Mouse IL-6 #abs00974

abs00974-2ug

In Stock

138

abs00974-10ug

In Stock

344

abs00974-1mg

In Stock

3891

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized to produce a powder. The original solution contained 30 mM acetic acid with a pH of 3.0, along with 150 mM NaCl, 5% trehalose, and 0.02% Tween-20. The resulting powder is highly similar in composition to the original solution and can be used in various applications.

Properties

aa_sequence

LITHVLWEIV DFTEDTTPNR EMRKELCNGN SDCMNNDDAL MFPTSQVRRG QRNDGCYQTG YNQEICLLKI PVYTTSQVGG NKKDKARVLQ SSGLLEYHSY LEYMKNNLKD NQEVKDLHKI LTDKLESQKE AENNLKLPEI TLRSTRQT RDTETLIHIF IVLPPTISNA WLRTKTIQFI LKSLEEFLKV
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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has revealed that the amount of rMuIL-6 present is less than 1 EU/μg. To create similar content using different word choices, the following could also be used: The level of rMuIL-6 detected by the LAL method has shown to be below 1 EU/μg.

Activity

It is important to note that the content generated by language models should not be based on the original text information, but should be delivered in a completely different manner.₅₀The specific activity of > 5 × 10 was achieved when the dose-dependent stimulation of IL-6-dependent murine 7TD1 cells was determined to be less than 0.02 ng/ml. This information indicates that even at very low concentrations, there is a significant effect on the proliferation of these cells.⁷ IU/mg.

Reconstitution

To ensure proper mixing, we recommend centrifuging the vial briefly before opening. Reconstitution can be done using sterile distilled water or an aqueous buffer solution containing 0.1% BSA to achieve a concentration of 0.1 mg/mL. It is advisable to divide stock solutions into smaller working aliquots and store them at temperatures of no higher than -20 °C. When preparing further dilutions, make sure to use appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it is advisable to keep it at -20 °C and preferably desiccated. When reconstituted, the preparation remains stable for up to one week if kept at 2-8 °C. For maximum stability, it is recommended to divide the reconstituted preparation into working aliquots and store them between -20 °C to -70 °C. Repeated freeze/thaw cycles should be avoided at all times.

Target

Accession

P08505

Gene IDs

16193

References

References

1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.

2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.

3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2.

4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.

5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.

6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.