Recombinant Human IL-5 #abs00807

abs00807-2ug

1-2 Weeks

138

abs00807-10ug

1-2 Weeks

344

abs00807-1mg

1-2 Weeks

5575

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20mM PB, pH 7.5, 3% trehalose, and 0.1% Tween-80 was filtered through a 0.2 μm filter. The resulting solution was then lyophilized to obtain a dried form.

Properties

aa_sequence

EEIDLM RNKQTGFNSA QEETTPGSRK SSLPTLKTQL IGQKTTINCD QLVTQRGVHR IFFGKLNYEY DEYKVHFNVP LELSATGIDG LGSFIGLKRV VLKNLKKVHI.EFITLTQTTS PQLLTEIENN QERNLTPHIS QGGNIIVEVC LVCYTQETTL VEGSKLRFLT MQRNTKKCYL SKYGVELNDF LERTEIEVGF.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determined amount of Recombinant Human IL-5 is less than 1 EU/μg, verified using the LAL method. To reiterate, the content above has been rearranged to maintain its original meaning.

Activity

The ED is equivalent in biological activity to the standard, making it fully functional.₅₀TF-1，0.1 ng/ml，1.0 × 10。。⁷ IU/mg.

Reconstitution

To ensure proper mixing, we advise giving this vial a brief centrifugation before opening. It is recommended to reconstitute the content with sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. For easy storage, divide the stock solutions into smaller working aliquots and keep them at a temperature of -20°C or lower. Any further dilutions should be performed using appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it should be kept at -20 °C and preferably desiccated. Once reconstituted, the preparation can be stored for one week at 2-8 °C. It is advised to divide the reconstituted preparation into working aliquots for optimal stability and store them at -20 °C to -70 °C. It is important to avoid repeated freeze/thaw cycles, as this can compromise the integrity of the preparation.

Target

Accession

P05113

Gene IDs

3567

References

References

1. MV Milburn, AM Hassell, MH Lambert, et al. 1993. Nature, 363: 172-6.

2. JS Lee, HD Campbell, CA Kozak, et al. 1989. Somat Cell Mol Genet, 15: 143-52.

3. BH van Leeuwen, ME Martinson, GC Webb, et al. 1989. Blood, 73: 1142-8.

4. S Dubucquoi, P Desreumaux, A Janin, et al. 1994. J Exp Med, 179: 703-8.