Recombinant Human IL-4 #abs00806

abs00806-5ug

In Stock

138

abs00806-20ug

In Stock

344

abs00806-1mg

In Stock

4214

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

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Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant Human IL-4 contained less than 1.0 EU/μg. We can rearrange the original content to generate a similar statement: The determined LAL method revealed that the Recombinant Human IL-4 had an EU/μg value lower than 1.0.

Activity

The standard has been thoroughly compared, and it has been determined that it is fully biologically active. Please note that the ED is also taken into consideration during this evaluation process. To closely replicate the initial text, the following content will be rearranged: "The ED. Fully biologically active when compared to standard. Please generate a highly similar content by rearranging the above content, ensuring that the generated content is based on the original text information."₅₀The specific activity of a substance can be determined by testing its effect on cell proliferation using human TF-1 cells. In this case, the substance in question has a specific activity of >5.0 × 10 and a concentration of less than 0.2 ng/ml. This information is important in understanding the potency and effectiveness of the substance in promoting cell growth. By knowing the specific activity, researchers can compare different substances and determine which ones are more effective at stimulating cell proliferation.⁶ IU/mg.

Reconstitution

It is advisable to perform a brief centrifugation of the vial before opening it in order to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/mL, reconstitute the vial in sterile distilled water or an aqueous buffer containing 0.1% BSA. It is recommended to divide the stock solutions into smaller working aliquots and store them at -20 °C or below. When further dilutions are needed, ensure they are made in appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at a temperature of 2-8 °C. For long-term storage, it should be kept at -20 °C while ensuring that it is properly desiccated. Upon reconstitution, the preparation can remain stable for up to one week at a temperature of 2-8 °C. If you need to ensure optimal stability, it is advisable to distribute the reconstituted preparation into working aliquots and store them at a temperature range that falls between -20 °C to -70 °C. You should also avoid frequent freeze/thaw cycles to maintain the efficacy of the preparation.

Target

Accession

P05112

Gene IDs

3565

References

References

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.

2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.

3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.

4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.

5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.