Recombinant Human TIGAR-TAT #abs01230

abs01230-5ug

1-2 Weeks

138

abs01230-25ug

1-2 Weeks

344

abs01230-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was first filtered through a 0.2 μm filter before being lyophilized. It had a composition of 30% acetonitrile and 0.1% TFA. To create a highly similar content, one could rearrange the information in this way.

Properties

aa_sequence

LKMTGZGVHE AASGVEDTRF LMDIAFGQIK EFAVTTNKSQ RKGFSNDLEM QEGVHITKAM RERKADRYSL TQEFLCAKDM RISVKYEGEV RMHAKAAERL PPVVTFSCRE KGDALHFMVR RVDLKELQKF EQLEKTVPIC RGFTSMLSED SKNVPHGALG LSASFLVVHY FAGDLYLFLR SSMPCATETL VLVTNSMTLE SFIGEENLLR GRIHTGDYVR KRNKQMKRQR RRETQGAE RGKYVETVVM R']].

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that Recombinant Human TIGAR-TAT has an EU/μg count of less than 0.1. This information can be rearranged to restate that the product has an extremely low endotoxin level, making it a safe and reliable option for medical and research usage.

Activity

After pretreating U2OS cells with Recombinant Human TIGAR-TAT for 4 hours, it was observed that the biological activity of the treated cells was fully biologically active when compared to the standard. To determine its effectiveness, the cells were exposed to hydrogen peroxide-induced apoptosis. The results showed that the concentration range of 0.1-5.0 μg/ml of Recombinant Human TIGAR-TAT was able to protect the U2OS cells from undergoing apoptosis. This data indicates that the treatment with Recombinant Human TIGAR-TAT has a significant impact on cell survival and provides a potential therapeutic option for mitigating apoptosis.

Reconstitution

Before opening this vial, it is recommended to briefly spin it in a centrifuge to ensure that the contents settle at the bottom. The reconstitution process requires sterile distilled water or aqueous buffer that contains 0.1 % BSA. This should be done to achieve a concentration range of 0.1-1.0 mg/ml. To store the stock solutions, it is advisable to aliquot them for ease of use, then freeze them at ≤ -20 °C. If further dilutions are necessary, this should be done using appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C without any issues, but for long term storage, it's recommended to keep it at -20 °C. It is best to store it in a desiccated state. Once reconstituted, the preparation remains stable for up to one week when refrigerated at 2-8 °C. To maintain its stability for a longer duration, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it can compromise its integrity.

Target

Accession

Q9NQ88

Gene IDs

57103