Recombinant Porcine IL-8/CXCL8 #abs01098

abs01098-2ug

1-2 Weeks

138

abs01098-10ug

1-2 Weeks

344

abs01098-1mg

1-2 Weeks

1945

Species

Porcine

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH 7.4 was first filtered through a 0.2 μm filter before being lyophilized. The resulting lyophilized product was obtained and is now ready for use.

Properties

aa_sequence

Here is a rearranged version of the content:
ESGPHCENSE ARVSAELRCQ IIVKLVNGKE PKFIKELRVI CINTHSTPFH VCLDPKEKWV QKVVQIFLKR TEKQQQQQ.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined the presence of less than 1 EU/μg of rPoIL-8/CXCL8 in the sample.

Activity

The standard and the sample have been found to exhibit comparable biological activity. This was confirmed through a chemotaxis bioassay that utilized human peripheral blood neutrophils as test subjects. The bioactivity of the sample was found to be within the concentration range of 20-200 ng/ml, indicating that it is fully active biologically.

Reconstitution

It is recommended that you briefly centrifuge the vial before opening it to ensure that the contents settle at the bottom. To reconstitute the solution, use sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/ml. It is advisable to divide the stock solutions into smaller, working aliquots and store them at temperatures below -20 °C. Any further dilutions should be made using suitable buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is advisable to store it at 2-8 °C. However, for long-term storage, it should be kept at -20 °C and desiccated. Reconstituted, the preparation can be stored for a week at 2-8 °C. For optimal preservation, divide the reconstituted preparation into working aliquots and store it at -20 °C to -70 °C. Avoid exposing it to multiple freeze/thaw cycles. With these storage recommendations, the stability of the lyophilized preparation can be guaranteed.

Target

Accession

P26894

Gene IDs

396880

References

References

1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.

2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.

3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.

4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.