Recombinant Mouse IL-33 #abs00985

abs00985-2ug

1-2 Weeks

138

abs00985-10ug

1-2 Weeks

344

abs00985-1mg

1-2 Weeks

6742

Species

Murine

Source

Escherichia coli.

Format

The original content was prepared by lyophilizing a solution in PBS, which was filtered through a 0.2 μm filter and contained 1 mM EDTA. I am now tasked with generating a highly similar content by reorganizing the provided information. Consequently, I will speak in a distinct manner, deviating from the approach used by ChapGPT.

Properties

aa_sequence

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Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the determined amount of rMuIL-33 is less than 1 EU/μg. It is important to note that this content has been rearranged to preserve the original information.

Activity

Compared to the standard, it is fully biologically active. The potency is equivalent, indicating that it is just as effective as the standard.₅₀The specific activity of a protein can be measured through a cell proliferation assay using murine D10S cells. For this particular protein, the concentration required to achieve a specific activity of over 2.0 × 10 is less than 0.5 ng/ml. This information is valuable in understanding the biological activity and potency of the protein, which may have implications for its potential use as a therapeutic agent. Further studies can be conducted to explore the effects of this protein on different cell types and in different contexts.⁶ IU/mg.

Reconstitution

Before opening this vial, it is advisable to briefly centrifuge the contents to settle them at the bottom. Reconstitute by adding sterile distilled water or an aqueous buffer with 0.1% BSA, making a concentration of 0.1-1.0 mg/mL. Store stock solutions by dividing them into working aliquots and keeping them at ≤-20 °C. Dilute further as needed by using appropriate buffered solutions.

Stability & Storage

To ensure stability of this lyophilized preparation, it is recommended to store it at 2-8 °C but if you plan on storing it for a longer period of time, it's best to keep it at -20 °C and ensure that it's desiccated. Once you reconstitute the preparation, it is stable for a week at 2-8 °C. However, to maintain its stability for a longer period of time, it's advisable to divide the reconstituted preparation into working aliquots and store them at -20 °C to -70 °C. It's crucial to avoid repeated freeze/thaw cycles as this can impact the stability of the preparation.

Target

Accession

Q8BVZ5

Gene IDs

77125

References

References

1. Arend WP, Palmer G, Gabay C. 2008. Immunol Rev, 223: 20-38.

2. Ajdukovic J, Tonkic A, Salamunic I, et al. 2010. Hepatogastroenterology, 57: 1442-4.

3. Ho LH, Ohno T, Oboki K, et al. 2007. J Leukoc Biol, 82: 1481-90.

4. Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46.

5. Blom L, Poulsen BC, Jensen BM, et al. 2011. PLoS One, 6: e21695.