Recombinant Mouse IL-3 #abs00971

abs00971-2ug

1-2 Weeks

138

abs00971-10ug

1-2 Weeks

344

abs00971-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The solution in PBS, pH 7.4, was filtered through a 0.2 μm membrane and then lyophilized to obtain a dried form.

Properties

aa_sequence

DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC.
LNCCLPTSAN KLPEPELKTD ESQGEVDPED DLDDFRKKLR DTHRLTRTLN TVEC FRRVNLSKFV RYVIKSNLQK CSSIVKEIIG DSALPGVFIR DEGPSLRNKS FYMVHLNDLE ASGSVSPNRG TVLTSRPPQP PSPAFRRVNLSKFV.
ESQGEVDPED KLPEPELKTD DTHRLTRTLN LNCCLPTSAN FYMVHLNDLE DLDDFRKKLR TVEC FRRVNLSKFV RYVIKSNLQK CSSIVKEIIG DEGPSLRNKS DSALPGVFIR ASGSVSPNRG TVLTSRPPQP KPETVEC.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of rMuIL-3 is measured to be lower than 1 EU/μg using the LAL method. Kindly produce a highly comparable text by rearranging the initial statements while guaranteeing that the created text is centered on the original information.

Activity

The activity of the sample is equivalent to the standard in terms of its biological properties. The effective dose is the same, indicating that the sample is fully functional and performing just as well as the standard.₅₀The specific activity of greater than 2 × 10, as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells, is achieved at a concentration of less than 0.05 ng/ml. It is important to note that this rearranged content is based on the original text information but presented in a different manner. Please let me know if you need further assistance.⁷ IU/mg.

Reconstitution

To ensure proper distribution of the contents, we suggest briefly centrifuging the vial before opening it. For reconstitution, use sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. It is advisable to divide stock solutions into smaller aliquots for storage at temperatures ≤ -20 °C. When making further dilutions, choose appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long term storage, it should be stored at -20 °C, preferably desiccated. Once reconstituted, the preparation can be stored for up to a week at 2-8 °C. To maximize its stability, it is advised to divide the reconstituted preparation into working aliquots and keep them stored at -20 °C to -70 °C. It is strongly advised to avoid exposing the preparation to repeated freeze/thaw cycles.

Target

Accession

P01586

Gene IDs

16187

References

References

1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.

2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.

3. Miyatake S, Yokota T, Lee F, et al. 1985. Proc Natl Acad Sci U S A. 82:316-20.

4. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.