Recombinant Mouse IL-2 #abs00970

abs00970-5ug

In Stock

138

abs00970-20ug

In Stock

344

abs00970-1mg

In Stock

3631

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized to obtain a powder. The lyophilized powder was prepared in a phosphate-buffered saline (PBS) solution with a pH of 7.4.

Properties

aa_sequence

LQEMDLLSLQ LNEYRMNRLK KFYLTFMRLP QDLEKLTAEQ QSSTTSSAPT QHQQQQQQQQ EQQHLQQQQQ VPLELDLEQC SKQLTDLHDV FENADELQSF KKVTVVRINS VEDTNSDGSL VTVVARINSK EDAVQFDDF LQAFCWIRRW IsFCTSPQSI.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determination of rMuIL-2 content using the LAL method reveals a result of less than 1 EU/μg. Let me generate highly similar content by rearranging the given information: "According to the LAL method, the content of rMuIL-2 is found to be less than 1 EU/μg."

Activity

Compared to the standard, this product is fully biologically active and provides similar effects. Its ED is identical to the original standard, making it a top-quality option.₅₀The specific activity of the protein, based on a cell proliferation assay using murine CTLL-2 cells, is greater than 5.0 × 10 and the concentration is less than 0.2 ng/ml. This information indicates a highly potent protein with a low concentration, which could have important implications in therapeutic applications.⁶ IU/mg.

Reconstitution

It is recommended to briefly centrifuge the vial before opening to ensure the contents are settled at the bottom. Reconstitution should be done using sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. After reconstitution, it is advisable to create working aliquots of the stock solution and store in ≤ -20°C. Dilution should be performed using appropriate buffered solutions.

Stability & Storage

For long term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated condition. However, it can be stored at 2-8 °C without any stability concerns. Upon reconstitution, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into separate working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as this can compromise its stability.

Target

Accession

P04351

Gene IDs

16183

References

References

1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.

2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.

3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.

4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.

5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.

6. Matesanz, F., A. Alcina, and A. Pellicer. 1993. Immunogenetics, 38: 300-3.