Recombinant Human IL-36β, 153a.a. #abs00833

abs00833-2ug

1-2 Weeks

138

abs00833-10ug

1-2 Weeks

344

abs00833-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 2× PBS, pH 7.4 was lyophilized after being filtered through a 0.2 μm membrane. Let's create a distinctive dialogue using a language model instead of generating content based on the original text information.

Properties

aa_sequence

VLWQSSMVDR GAISLNSAPR IRKTLVPSIS EFSTDKYCAL VYMNGKEKLD LCFDLKGGKI LTPIQGEAKQ DMINKKELEY PFQGKKAQEV TGSLFSTNSI GYPVSQVSFW TGSTTAIFWP RGEKTLFIQN DYFNNNTTIS EVS.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human IL-36β, 153a.a. has been determined to be lower than 1 EU/μg using the LAL method. Please create an alternative version of the text, ensuring that it is based on the original information provided.

Activity

The ED is comparable to the standard in terms of its full biological activity.₅₀The specific activity of inducing IL-8 secretion in human preadipocytes is greater than 1.0 × 10, as determined by measuring a secretion level of less than 10 ng/ml.⁵ IU/mg.

Reconstitution

To ensure the contents are properly settled, it is recommended to briefly centrifuge the vial before opening. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/ml. It is advisable to divide the stock solution into smaller aliquots for immediate use and store them at -20°C or below. Dilutions can be made in suitable buffered solutions as needed.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8°C. However, to ensure long-term storage, it is advisable to keep it at -20°C and preferably in a desiccated condition. When reconstituted, the preparation can remain stable for a week if kept at 2-8°C. It is best to divide the reconstituted preparation into smaller working aliquots and store them at -20°C to -70°C for maximum stability. Avoid exposing the preparation to repeated freeze/thaw cycles, which can affect its stability.

Target

Accession

Q9NZH7-2

Gene IDs

27177

References

References

1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25.

2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973.

3. Magne D, Palmer G, Barton JL, et al. 2006. Arthritis Res Ther. 8:R80.

4. Van Asseldonk EJ, Stienstra R, Koenen TB, et al. 2010. Obesity (Silver Spring). 18:2234-6.

5. Johnston A, Xing X, Guzman AM, et al. 2011. J Immunol. 186:2613-22.