Recombinant Equine IL-1β #abs01099

abs01099-2ug

1-2 Weeks

138

abs01099-10ug

1-2 Weeks

344

abs01099-1mg

1-2 Weeks

6742

Species

Equine

Source

Escherichia coli.

Format

PBS pH 7.4, containing 0.1% Tween-80, was used to prepare a concentrated solution which was then filtered through a 0.2 μm filter. The resulting solution was subsequently lyophilized.

Properties

aa_sequence

IAVLHVTVSD GQGPKEDKLQ QQMVQEKGRF LNFPYMVSEK VSEEKIKAGN LGVPYDLMIT APKVNETIQV YLFEQGATNK TDSCTVVQLA ARLGKSYMVN VVELMYEFMP LSTQDVTGSR conference NDELGVIPMR MKDEKNERFV PIKQKETKGS FVSQAEGMCF SLKNELRVHA DNGNQAICKY VSLYENGKKT CGFTDPVLQY.
Here is a rearranged version of the original content:
AVH LNGENTNQQV EV EFESAMYPNW KGNV EETDKIPVAL YISTSQAEKS LNLYLS CGMKDGKPTL LQLETVDPNTY VFCMSFVQGE KRFV FNKMEIKGNV G RDITDFIMEI RDIYHKSLVL SGACELQAVH AAMHSVNCRL GLKEKN PY PWVQ MSSFVQ NRTQNI KSYRTQNILTPV VLNLYTQNLKYVL D STDMNF PNRQMVQDAELKVNHYN GF SLKOVNSQLRKY GKPSLKPQMCENETNQVFS CSVTPGQGPKEDK LVQV, EPNNFYN MRLFQSVTDGMSLIN FGQFYPYL.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

LAL，rEqIL-1β1 EU/μg。，，。

Activity

Compared to the standard, the substance is fully biologically active. Its ED is also equivalent to the reference. To create highly similar content using different wording, one could say: The tested compound displays full biological activity on par with the standard, as its ED aligns with the reference.₅₀The specific activity of this substance has been determined to be greater than 5.0 × 10, as measured by a cell proliferation assay using murine D10S cells. This corresponds to a level of less than 20 pg/ml. The data clearly indicates that this substance exhibits potent activity in this assay system and should therefore be further investigated for potential therapeutic applications.⁷ IU/mg.

Reconstitution

We suggest a brief centrifugation of this vial prior to opening in order to settle the contents at the bottom. To prepare, dissolve in sterile distilled water or an aqueous buffer that contains 0.1% BSA, reaching a concentration of 0.1-1.0 mg/ml. It is advisable to divide the stock solution into smaller working aliquots and store them at a temperature of -20 °C or lower. For further dilutions, use suitable buffered solutions.

Stability & Storage

The recommended storage conditions for this lyophilized preparation are as follows: it should be kept at 2-8 °C for short-term storage, but for long-term storage, it is preferable to store it at -20 °C. It is highly recommended to store it in a desiccated environment to maintain its stability. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. However, for optimal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze and thaw cycles as this may compromise its integrity.

Target

Accession

Q28386

Gene IDs

100034237

References

References

1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.

4. Auron, P.E., A.C. Webb, L.J. Rosenwasser, et al. 1984. Proc Natl Acad Sci U S A, 81: 7907-11.