Recombinant Porcine IL-2 #abs01097

abs01097-2ug

1-2 Weeks

138

abs01097-10ug

1-2 Weeks

344

abs01097-1mg

1-2 Weeks

5058

Species

Porcine

Source

Escherichia coli.

Format

The concentrated solution in 1 × PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to obtain the final product. The resulting lyophilized powder was carefully prepared to ensure that it retained the original properties of the concentrated solution. The final product is highly pure and suitable for a wide range of applications. The process of lyophilization ensures that the product is stable and can be stored for long periods of time without any degradation in quality. Overall, this is a reliable and efficient method for preparing high-quality products for research and other applications.

Properties

aa_sequence

VLEELKALEG KGSNNDSQSA NIKESMNNI EFLNDSKTSF TFCQSIYEKL QYADLSRMLN KHLQCLVETL CSLLQLEPLL FKFYMPKQAA NNITVLELKR ETSAMPTSKN NLGQSNMQTS DVEYENALSR STLTQEVKKG KNTLNQQACS DFLYTLKC.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the amount of rPoIL-2 is less than 1 EU/μg. It is important to note that the content generated should be based on the original text information but presented in a highly similar manner.

Activity

The standard's full biological activity has been matched by the ED. To produce similar content, the meaning of the original text can be paraphrased as follows: The ED has been found to be fully biologically active, with potency equivalent to the standard.₅₀The specific activity of a certain substance, as measured by a cell proliferation assay using mouse CTLL-2 cells, is remarkably high. It is less than 0.5 ng/ml, which indicates a specific activity of greater than 2.0 × 10. This measurement provides important information about the potency and efficacy of this substance in inducing cell growth and proliferation. Additionally, it is a valuable tool for researchers seeking to understand the biological mechanisms underlying cell proliferation and related processes.⁶ IU/mg.

Reconstitution

To ensure optimal results, we suggest centrifuging this vial briefly before opening to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. Prepare stock solutions in small working aliquots and store them at or below -20°C. Dilute further as needed in appropriate buffered solutions. By following these guidelines, you can ensure the best possible outcome for your research.

Stability & Storage

The recommended storage conditions for this lyophilized preparation are as follows: it should be kept at a temperature range of 2-8 °C for short-term stability, while for long-term storage, it is advisable to store it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at temperatures of 2-8 °C. However, for maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may affect its stability.

Target

Accession

P26891

Gene IDs

396868

References

References

1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.

2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.

3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.

4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.

5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.