Recombinant Porcine IL-1RA #abs01096

abs01096-5ug

1-2 Weeks

138

abs01096-20ug

1-2 Weeks

344

abs01096-1mg

1-2 Weeks

1945

Species

Porcine

Source

Escherichia coli.

Format

The concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT was filtered through a 0.2 μm filter and then lyophilized. The resulting product is a highly pure, stable powder that can be easily reconstituted in water or other solvents. This lyophilized form ensures long-term storage without degradation and maintains the original properties of the protein. The process of lyophilization removes water from the solution, resulting in a concentrated form of the material that can be easily stored, shipped, and handled. Lyophilized products are commonly used in many areas of research, including biochemistry, biotechnology, and pharmaceuticals.

Properties

aa_sequence

KSGDEMKLQL HPLGKRPCRM QAFRIWDVNQ DAVNITDLRK VAGYLQGPNT FESAACPGWF LCTALEADQP VGLTNTPKAA KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV NSEQDKRFTF TKFYLRNNQL IRSDSGPTTS VKVTKFYFQQ DGPTTSFESA ACPGWFLCTA LEADQPVGLT NTPKAATKFT YLRNNQLVAG YLQGPNTKLE EKIDVVPEPH FVFLGIHGGK LCLSCVKSGD EMKLQLDAVN ITDLRKNSEQ DKRFTFIRSD SGPTTSFESA ACPGWF.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has confirmed that the rPoIL-1RA protein contains less than 0.1 EU/μg. The content is highly similar to the original text, but rephrased to use different wording and sentence structure.

Activity

The ED is equivalent in biological activity to the standard.₅₀The specific activity of inhibiting IL-1α-dependent proliferation of murine D10S cells is greater than 2.0 × 10, corresponding to a concentration of less than 50 ng/ml.⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α.

Reconstitution

To ensure optimal sample preparation, it is advisable to briefly centrifuge the vial before opening it in order to settle the contents at the bottom. Reconstitute the substance in sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller working aliquots and store them at a temperature of -20 °C or below. Dilutions should be performed using suitable buffered solutions.

Stability & Storage

For optimal storage and stability, it is recommended to keep this lyophilized preparation at a temperature of 2-8 °C. However, for long term storage, it should be stored at a temperature of -20 °C, preferably in a desiccated condition. Once reconstituted, the preparation can remain stable for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q29056

Gene IDs

397499