Recombinant Mouse HB-EGF #abs01018

abs01018-10ug

1-2 Weeks

138

abs01018-50ug

1-2 Weeks

344

abs01018-1mg

1-2 Weeks

1945

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 10 mM PB, 500 mM NaCl, pH7.4 was filtered through a 0.2 μm membrane and then lyophilized. You can expect a highly similar product generated from this same material.

Properties

aa_sequence

CRHYGHGPLC SCTPREFQYL RCEGHICYDK YKRCLPRDKK GLGKGKKKKK GNREKSPATL KQPSFSAVKF LNLDTGLKVE SSLSPYENVG FDSPNITRGD VTEHGKKLRL TGYSVFVRCG.
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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determination of rMuHB-EGF using the LAL method indicates that it contains less than 1 EU/μg.

Activity

Compared to the standard, the biological activity of this product is fully intact. The effective dose is equivalent to that of the original.₅₀The specific activity of this substance, as determined by a cell proliferation assay using murine Balb/c 3T3 cells, is greater than 1.0 × 10 and the concentration required to elicit this activity is less than 1 ng/ml. This information underscores the potency and efficacy of the substance, as it demonstrates that only very small amounts are needed to induce biological activity.⁶ IU/mg.

Reconstitution

Before opening the vial, it is recommended to briefly centrifuge it so that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/ml. It is advisable to divide stock solutions into working aliquots and store them at a temperature of -20 °C or below. For further dilutions, use appropriate buffered solutions.

Stability & Storage

To ensure optimal storage conditions for this lyophilized preparation, it is recommended to keep it at -20°C for long term storage, preferably in a desiccated form. Although it is stable at 2-8°C, for maximum stability, it is advised to apportion the reconstituted preparation into working aliquots and store them at -20°C to -70°C. It is important to avoid repeated freeze/thaw cycles as it may affect the quality of the preparation. Upon reconstitution, it can be stored for up to one week at 2-8°C. Overall, proper storage and handling of this preparation is crucial to ensure its effectiveness when used in experiments or procedures.

Target

Accession

Q06186

Gene IDs

15200

References

References

1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.

2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.