Recombinant Human HB-EGF #abs00905

abs00905-10ug

1-2 Weeks

138

abs00905-50ug

1-2 Weeks

344

abs00905-1mg

1-2 Weeks

1945

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl was filtered through a 0.2 μm membrane and subsequently lyophilized.

Properties

aa_sequence

SCKYDFCIHG ECKYVKELRA PSCICHPGYH GERCHGLSL DLQEADLDLL RVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK.
The above content contains a sequence of letters and numbers. It appears to be a code or encrypted message. However, with the right skills and knowledge, it can be deciphered and translated into a meaningful text. Perhaps, it holds a secret message, an important announcement, or simply a fun puzzle to solve. Whatever the case may be, it is up to the reader to unravel the mystery and unlock the hidden meaning behind these characters.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that Recombinant Human HB-EGF contains less than 1 EU/μg. This indicates a high level of purity and quality.

Activity

The standard has been surpassed by the fully biologically active ED. The level of similarity between the two is remarkable.₅₀The cell proliferation assay using murine Balb/c 3T3 cells revealed that the concentration required for activity is less than 1 ng/ml, indicating a specific activity higher than 1.0 × 10.⁶ IU/mg.

Reconstitution

To prepare this vial for use, we suggest a brief centrifugation prior to opening to ensure the contents settle to the bottom. Reconstitution may be achieved using sterile distilled water or an aqueous buffer containing 0.1% BSA to create a solution with a concentration of 0.1 mg/ml. Stock solutions should be divided into working aliquots and stored at a temperature of -20°C or lower. For further dilution, appropriate buffered solutions should be utilized. This process will ensure optimal results and efficacy.

Stability & Storage

For optimal storage conditions, it is recommended to keep this lyophilized preparation at -20 °C with desiccation. However, it can be stored at 2-8 °C without any issue. Once reconstituted, it remains stable for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller aliquots and store them at -20 °C to -70 °C. It is important to avoid subjecting the preparation to repetitive freeze/thaw cycles.

Target

Accession

Q99075

Gene IDs

1839

References

References

1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.

2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.