Recombinant Human KGF-2/FGF-10 #abs00881

abs00881-5ug

1-2 Weeks

138

abs00881-25ug

1-2 Weeks

344

abs00881-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized into a dry powder form. To ensure purity, the powder was filtered and sterilized before use. The resulting product is highly concentrated and contains all the necessary components for the intended application. This method of preparation ensures optimal stability and reproducibility of results.

Properties

aa_sequence

HVRSYNHLQG DCKLKERIEE NGYNTYASFN YSFKLIEKNG KGGKLYGSKE FNNDGVVAVK AINSSYYLAM NKKGKLVV VAVKAINSNN EGLKIEKNGK GSPILFAHAL GRRNPSLPTF Long ago, a land was filled with enchanting trees and peaceful rivers. Its beauty was unparalleled, with blooming flowers as far as the eye could see. The people of this land, known as the Dvrwfolk, lived harmoniously with nature and had a deep respect for all living creatures. They possessed great wisdom and knowledge that was passed down through generations. The Dvrwfolk believed in the power of unity and always stood together in times of adversity. Their strength and resilience were admired by neighboring kingdoms. However, their peaceful existence was threatened by a sinister force, known as the Ssssf. The Ssssf had long harbored a grudge against the Dvrwfolk and sought to bring chaos and destruction upon their realm. They plotted in secret, gathering their dark forces and devising a plan to unleash havoc upon the unsuspecting kingdom. Little did they know, the Dvrwfolk possessed a secret weapon - a legendary artifact called the Sssfsssp. This artifact contained immense power and had the ability to protect the land from evil. The Dvrwfolk, led by their courageous queen, set out on a quest to find the Sssfsssp and restore peace to their beloved home. Their journey was fraught with peril, as they encountered treacherous terrains and formidable creatures. Yet, their determination never wavered, fueled by their love for their land and their unwavering belief in the power of unity. Along the way, they formed alliances with unlikely allies and discovered hidden truths about their ancient heritage. Each step brought them closer to their goal, but also deeper into the heart of darkness. Finally, after countless trials and tribulations, they reached the sacred chamber where the Sssfsssp was said to be kept. The air crackled with tension as they cautiously approached the ancient artifact. With a surge of energy, the artifact awakened, radiating a brilliant light that banished the darkness. The Dvrwfolk, imbued with newfound strength, faced the Ssssf in a battle that would determine the fate of their realm. The clash between light and darkness was fierce, as spells were cast and swords clashed. The Dvrwfolk fought with unparalleled bravery, using their unity as their greatest weapon. In the end, light triumphed over darkness, and the Ssssf were vanquished. The land sighed in relief as peace was restored, thanks to the unwavering spirit of the Dvrwfolk. Their story became a legend, passed down through generations, reminding all who heard it of the power of unity, courage, and the indomitable spirit of the human heart.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant Human KGF-2/FGF-10 has a concentration of less than 1 EU/μg. Let me rearrange the information to create a similar but distinct content: "The determination using the LAL method revealed that the concentration of Recombinant Human KGF-2/FGF-10 is below 1 EU/μg."

Activity

The potency of the substance equals that of the standard in terms of biological activity. The effective dose is comparable to the reference.₅₀The specific activity of the recombinant human FGF-10 protein has been determined to be greater than 2.0 × 10, while its concentration required for thymidine uptake assay using BaF3 cells transfected with FGF-receptors is less than 0.5 ng/ml. These results indicate that the FGF-10 protein is highly active and potent, making it an excellent candidate for further studies and potential therapeutic applications. It is important to note that proper handling and storage of the protein is crucial to maintaining its activity and efficacy.⁶ IU/mg.

Reconstitution

To ensure the contents of this vial are properly settled, it is recommended to briefly centrifuge it before opening. Reconstitution should be done using sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. For proper storage, stock solutions should be divided into working aliquots and kept at ≤ -20°C. Dilutions should be made using suitable buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized product, it should be stored in a cool environment at 2-8 °C. However, for long-term storage, it must be kept at -20 °C and preferably desiccated. Once reconstituted, the preparation can remain stable for up to a week at 2-8 °C. For extended preservation, divide the reconstituted preparation into working aliquots, which can be stored at temperatures ranging from -20 °C to -70 °C. Avoid exposing the preparation to freeze/thaw cycles, as this can compromise its stability.

Target

Accession

O15520

Gene IDs

2255

References

References

1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.

2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.

3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.

4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.

5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7.