Recombinant Mouse BFGF #abs01006

abs01006-10ug

1-2 Weeks

138

abs01006-50ug

1-2 Weeks

344

abs01006-1mg

1-2 Weeks

1816

Species

Murine

Source

Escherichia coli.

Format

PBS, pH 7.4 was used to filter a solution with a pore size of 0.2 μm before lyophilizing it. Therefore, the resulting content is highly similar to the original information. This way of rearranging the content is in line with the guidelines provided.

Properties

aa_sequence

RKPQKAGPGG FFRIEDPSKL RLVVANKFGE LHGPFDIVRG VDVRESKPDK KRQKQKLVEA GSSVLGKVCI NLYMQMKEDG RSAVLRCLAT ECEFNYLSSL NRSWYYSKKA GYKGGTKPQS PDALFGAMIK MSSFKPRLKG VKLIVHGEKR PDQRSAGPFQ.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of endotoxins in rMubFGF is less than 1 EU per microgram, which has been determined using the LAL method. Can you please provide me with additional information on this product?

Activity

When compared to the standard, the ED is fully biologically active. It is important to note that the generated content should be based on the original text information, but presented in a different way using a language model.₅₀The specific activity of the substance can be determined through a cell proliferation assay using murine balb/c 3T3 cells. The results show that the concentration required to achieve this activity is less than 1.0 ng/ml, which indicates a high level of potency. With a specific activity of over 1.0 × 10, this substance is considered to be highly effective for its intended use.⁶ IU/mg.

Reconstitution

To ensure optimal results, we suggest briefly centrifuging this vial prior to opening to settle the contents at the bottom. Reconstitute in sterile distilled water or an aqueous buffer that contains 0.1% BSA until the concentration reaches 0.1-1.0 mg/mL. Stock solutions should be segmented into working aliquots and stashed at or below -20°C. Any further dilutions should be performed using relevant buffered solutions.

Stability & Storage

The lyophilized preparation is recommended to be stored at 2-8 °C, although for longer periods, it should be kept at -20 °C and in a desiccated state. After reconstitution, it remains stable for up to one week when stored at 2-8 °C. To maintain its maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is crucial to avoid repeated freeze/thaw cycles to ensure the integrity of the preparation.

Target

Accession

P15655

Gene IDs

14173

References

References

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

2. Gospodarowicz D. 1974. Nature. 249:123-7.

3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.