Recombinant Human FGF-9 #abs00880

abs00880-5ug

1-2 Weeks

138

abs00880-20ug

1-2 Weeks

344

abs00880-1mg

1-2 Weeks

5058

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

LYPDNVGEAF FVPGNVADAV VPLPVSDVLP GHSLQDLEGS AGPGARLLVD GILKLDHLDL YRQCLRGLFH PNIIEFGTQR KSHDRITGQG FIISALLEFI RNVSIDGLVG MSGDNKYLEL KTEGSLEYGE FERNFQEWYC TNYNSLHVDT RRGYAVLKDN GTRHKERPTF LPHPKFVDVD KLYPQDSIIL SQQMT。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined the amount of Recombinant HumanFGF-9 to be less than 1 EU/μg. Please rearrange the information to generate a highly similar content.

Activity

The standard and the compared item have identical biological activity. The potency is completely equivalent.₅₀The specific activity of less than 0.5 ng/ml, as assessed by thymidine uptake assay using BaF3 cells transfected with FGF-receptors, indicates a highly potent effect. This finding suggests that the tested substance exhibits a strong ability to stimulate cell growth mediated by FGF-receptors. The remarkably high specific activity, exceeding 2.0 × 10, further emphasizes the potency of the substance in promoting cellular proliferation through FGF signaling pathways. These results confirm the significant bioactivity and effectiveness of the substance in activating FGF-receptors and promoting cell growth.⁶ IU/mg.

Reconstitution

Before opening the vial, we highly recommend briefly centrifuging it to ensure that the contents settle at the bottom. To reconstitute, use 1 × PBS and achieve a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into smaller working aliquots and store them at temperatures at or below -20 °C. If further dilutions are necessary, make sure to use appropriate buffered solutions.

Stability & Storage

For optimal storage, it is recommended to keep this lyophilized preparation at -20 °C with desiccation. However, short-term storage at 2-8 °C is also acceptable. When reconstituted, the preparation remains stable for one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. To maintain the integrity of the product, avoid subjecting it to repeated freeze/thaw cycles.

Accession

P31371

Gene IDs

2254

References

References

1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.

2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.

3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.