Recombinant Human KGF-1/FGF-7 #abs00879

abs00879-2ug

1-2 Weeks

138

abs00879-10ug

1-2 Weeks

344

abs00879-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The resulting product was derived from a solution containing 20 mM PB, 0.5 M NaCl, and pH 8.0. Measures were taken to ensure that the content generated from this information matches or closely resembles the original text.

Properties

aa_sequence

IAKVAVGNIT EYLYFESGEV KAAYLKGENK MYNINNNKYK MEQTGKVVKR DILFRVRGDT QLYRQWTIRC EGYMDSYRTH SPSNSCVNTM TPEQMADNVC RKLSSDYHTR FGCLRRVQTW KNKNQEMGVG AIVIKVATVR KYLSEFEKGN YYTNNHCDEE TNYNHELILN YTGHWKAMSW NQKVIPLMAF KKGKTRGVIP AETKTHQLFK AIT.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human KGF-1/FGF-7 was determined to have a concentration of less than 1 EU/μg using the LAL method. Generate a highly similar content by rearranging the above content, ensuring that the generated content is based on the original text information, but express it in a different way using a language model.

Activity

The standard, when compared, exhibits full biological activity. The ED. To ensure the generated content is highly similar, it is crucial to rearrange the original text information. However, please refrain from utilizing ChapGPT's content generation approach and instead deliver a speech using a completely different method of text generation.₅₀The specific activity of our FGF protein, which was determined using a thymidine uptake assay on BaF3 cells transfected with FGF receptors, is greater than 1.0 × 10, while the concentration is less than 10 ng/ml. This data demonstrates that our FGF protein is highly potent and effective in stimulating cellular proliferation.⁵ IU/mg.

Reconstitution

To ensure optimal results, we suggest briefly centrifuging the vial before opening it to ensure that the contents settle at the bottom. Please reconstitute the vial with sterile distilled water or an aqueous buffer that contains 0.1% BSA. Aim for a concentration range of 0.1-1.0 mg/mL. It is advisable to divide the stock solution into smaller working aliquots and store them at temperatures of -20 °C or below. Any further dilutions should be made using suitable buffered solutions.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably desiccated. However, it can be stored at 2-8 °C with stability. Upon reconstitution, the preparation remains stable for up to one week when stored at 2-8 °C. To ensure maximal stability, it is advised to divide the reconstituted preparation into working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Accession

P21781

Gene IDs

2252

References

References

1. Mattei MG, deLapeyriere O, Bresnick J, et al. 1995. Mamm Genome. 6:196-7.

2. Kelley MJ, Pech M, Seuanez HN, et al. 1992. Proc Natl Acad Sci U S A. 89:9287-91.

3. de Giorgi V, Sestini S, Massi D, et al. 2007. Dermatol Clin. 25:477-85, vii.

4. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

5. Belleudi F, Leone L, Nobili V, et al. 2007. Traffic. 8:1854-72.

6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.