Recombinant Rat BFGF #abs01071

abs01071-10ug

1-2 Weeks

138

abs01071-50ug

1-2 Weeks

344

abs01071-1mg

1-2 Weeks

1816

Species

Rat

Source

Escherichia coli.

Format

The solution in PBS with a pH of 7.4 was filtered through a 0.2 μm filter and then lyophilized to preserve it.

Properties

aa_sequence

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Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

LAL，rRtbFGF1 EU/μg。，。

Activity

The potency of the product is the same as the standard, indicating full biological activity.₅₀The specific activity of the cell proliferation assay using murine balb/c 3T3 cells is > 5.0 × 10, indicating that the concentration required for significant cell growth inhibition is less than 0.2 ng/ml.⁶ IU/mg.

Reconstitution

Before opening the vial, it is recommended to briefly centrifuge it in order to settle the contents to the bottom. Next, reconstitute the contents in sterile distilled water or an aqueous buffer that contains 0.1 % BSA, to achieve a concentration of 0.1-1.0 mg/ml. Stock solutions should then be divided into working aliquots and stored at a temperature of ≤ -20°C. If further dilutions are needed, they must be made in appropriate buffered solutions. It is imperative to ensure that the generated content is based on the original text information, but is produced using language modelling in order to create text that is highly similar, yet different from the original wording.

Stability & Storage

It is recommended to store this lyophilized preparation at -20 °C for long-term storage, preferably in a desiccated condition. However, it can also be stored at 2-8 °C with stability. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P13109

Gene IDs

54250

References

References

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

2. Gospodarowicz D. 1974. Nature. 249:123-7.

3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.