Recombinant Human BMP-2 #abs00937

abs00937-2ug

1-2 Weeks

138

abs00937-10ug

1-2 Weeks

344

abs00937-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution, pH 3.5, containing 10 mM sodium citrate was subjected to filtration through a 0.2 μm filter and subsequently lyophilized.

Properties

aa_sequence

LYLDENEKVV DWIVAPPGYH KACCVPTELS AISMLKSSCK RHPLAFYCHG ECPFVNSKIP KNHAIIVQTL VNSYVDFSDV GWNPLADHLN STNGCGCRMQ AKHKQRKRNY QDMVVE

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the amount of Recombinant Human BMP-2 is below 1 EU/μg. Please generate a new content that is completely different from the original text and is based on the given information.

Activity

The standard is considered the benchmark for full biological activity. The ED is comparable to the standard in terms of its biological activity. To emphasize the similarity, please rearrange the content accordingly.₅₀The specific activity of inducing alkaline phosphatase production in murine ATDC5 cells should be greater than 5.0 × 10, with a corresponding level of less than 200 ng/ml. This information is important when studying the effects of various compounds on cell differentiation and bone formation. By carefully monitoring these levels, researchers can gain a better understanding of how certain substances interact with cells and potentially develop new treatments for bone-related disorders. Overall, precise measurements and calculations are crucial for advancing our knowledge of cell biology and related fields.³ IU/mg.

Reconstitution

Before opening the vial, we suggest briefly centrifuging it so that the contents settle at the bottom. To prepare, reconstitute the vial with sterile distilled H2O at a concentration of 0.1-1.0 mg/mL. For long-term storage, divide the stock solution into smaller aliquots and store them at -20 °C or lower. For further dilutions, use appropriate buffered solutions, and avoid reconstituting in PBS or cell culture media.

Stability & Storage

It is recommended to store this lyophilized preparation at a temperature range of 2-8 °C, but for long term storage, it should be kept at -20 °C. It is advisable to ensure that the preparation is desiccated during storage. Upon reconstitution, the prepared solution remains stable for one week when stored at 2-8 °C. To maintain its stability, it is best to divide the reconstituted preparation into smaller working aliquots and store them at temperatures between -20 °C and -70 °C. Make sure to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P12643

Gene IDs

650

References

References

1. Chung YI, Ahn KM, Jeon SH, et al. 2007. J Control Release, 121: 91-9.

2. Yamaji K, Kawanami M, Matsumoto A, et al. 2007. Dent Mater J, 26: 481-6.

3. Allen RT, Lee YP, Stimson E, et al. 2007. Spine (Phila Pa 1976), 32: 2996-3006.

4. Shen B, Wei A, Tao H, et al. 2009. Tissue Eng Part A, 15: 1311-20.

5. Seib FP, Lanfer B, Bornhauser M, et al. 2010. J Tissue Eng Regen Med, 4: 324-7.

6. Itoh T, Ando M, Tsukamasa Y, et al. 2012. FEBS Lett, 586: 1693-701.