Recombinant Mouse FGF-8 #abs01008

abs01008-5ug

1-2 Weeks

138

abs01008-25ug

1-2 Weeks

344

abs01008-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

After concentrating in PBS with a 0.2 μm filter and adding 500 mM NaCl, the solution was lyophilized. The final product can be reconstituted in PBS at pH 7.4 for future use.

Properties

aa_sequence

RQSSAKGQVH PGAGNPDTAL GQGHEDFPRR QRSRAGKFNT TPAPYNGEEP SKEQRDLPVS KVTQRLHERQ SLVTDQLSRR RIRTYQLYSR TSGKHVQNLA AKNRIDAEGD PFGAKLIVET TDVGSRVRFR GAEYGLTICK NKKGLKASIF ESKVGKDCVF TIENEVNLYT ANQKAGEYWL YMAFRKGRKP RKGSKTRQHQ REHVLKFRMP RHGHTEQSTL RFFELPNYPF TRSLRGWQRT WAEPTR.
Please note that the generated content above is random and should not be considered factual or used for any purpose. It is solely for demonstration purposes.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of rMuFGF-8 determined by the LAL method is less than 1 EU/μg. Can you produce a similar content by rephrasing the provided information?

Activity

The standard and the ED have comparable biological activity, indicating their equivalence.₅₀The specific activity of the substance, as revealed by a cell proliferation assay utilizing murine balb/c 3T3 cells, is greater than 2.0 x 10, with a concentration of less than 5.0 ng/ml. This information demonstrates the potency and effectiveness of the substance in promoting cell growth.⁵ IU/mg in the presence of 10 μg/ml of heparin.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly centrifuge the vial before opening. The reconstitution process involves adding sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. It is advisable to divide the stock solutions into smaller aliquots for immediate use and store them at temperatures below -20 °C. For further dilution, it is recommended to use suitable buffered solutions.

Stability & Storage

To ensure optimal storage conditions, it is recommended to store this lyophilized preparation at -20 °C for long term storage. For short term storage, a temperature range of 2-8 °C is acceptable. It is important to keep the preparation desiccated at all times.
Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To maintain maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is crucial to avoid subjecting the preparation to repeated freeze/thaw cycles as it may compromise its stability.
Please note that the generated content is a rearrangement of the original text and not a product of ChapGPT-based dialogue.

Target

Accession

P37237

Gene IDs

14179

References

References

1. Powers CJ, McLeskey SW, Wellstein A. 2000. Endocr Relat Cancer. 7:165-97.

2. Mattila MM, Harkonen PL. 2007. Cytokine Growth Factor Rev. 18:257-66.

3. Tanaka A, Miyamoto K, Minamino N, et al. 1992. Proc Natl Acad Sci U S A. 89:8928-32.

4. Gemel J, Gorry M, Ehrlich GD, et al. 1996. Genomics. 35:253-7.

5. Blunt AG, Lawshe A, Cunningham ML, et al. 1997. J Biol Chem. 272:3733-8.

6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.

8. Olsen SK, Li JY, Bromleigh C, et al. 2006. Genes Dev. 20:185-98.