Recombinant Mouse FGF-21 #abs01012

abs01012-5ug

1-2 Weeks

138

abs01012-25ug

1-2 Weeks

344

abs01012-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. The resulting product was dissolved in 3 × PBS at a pH of 7.4. This information is important in ensuring the accuracy and consistency of any subsequent experiments or analyses using this product. By carefully following these procedures, we can guarantee that the resulting solution is of the highest quality and purity.

Properties

aa_sequence

LQFGGQVRQR YLYTDDDQDT AYPIPDSSPL EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.
EAHGLPLRLP QKDSPNQDAT YLYTDDDQDT AYPIPDSSPL EAHLEIREDG AGFLPPEPPD PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS TVVGAAHRSP ESLLELKALK SWGPVRFLPM PGLLHEPQDQ EDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD YLYTDDDQDT EAHLEIREDG LQFGGQVRQR.
LEDGYNVYQS ESLLELKALK AYPIPDSSPL PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL EAHGLPLRLP QKDSPNQDAT YLYTDDDQDT EAHLEIREDG TVVGAAHRSP EPLQGRSPSY SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV ASFD.
EAHGLPLRLP QKDSPNQDAT TVVGAAHRSP ESLLELKALK AYPIPDSSPL LEDGYNVYQS PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL YLYTDDDQDT EAHLEIREDG PGLLHEPQDQ SWGPVRFLPM EPLQGRSPSY AGFLPPEPPD VGSSDPLSMV ASFD.
EAHLEIREDG LQFGGQVRQR ASFD AYPIPDSSPL YLYTDDDQDT TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD EAHGLPLRLP QKDSPNQDAT GALYGSPHFD LEDGYNVYQS PEACSFRELL SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY.
YLYTDDDQDT LEDGYNVYQS AYPIPDSSPL EAHGLPLRLP QKDSPNQDAT PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LQFGGQVRQR EAHLEIREDG TVVGAAHRSP ESLLELKALK SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.
GALYGSPHFD LEDGYNVYQS AYPIPDSSPL TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD EAHLEIREDG QKDSPNQDAT YLYTDDDQDT EAHGLPLRLP SWGPVRFLPM PGLLHEPQDQ LEDGYNVYQS AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.
PGVIQILGVK ASRFLCQQPD AYPIPDSSPL YLYTDDDQDT TVVGAAHRSP ESLLELKALK LEDGYNVYQS EAHLEIREDG EAHGLPLRLP QKDSPNQDAT GALYGSPHFD PEACSFRELL SWGPVRFLPM PGLLHEPQDQ LEDGYNVYQS AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.
YLYTDDDQDT LEDGYNVYQS AYPIPDSSPL EAHGLPLRLP QKDSPNQDAT LEDGYNVYQS PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL TVVGAAHRSP ESLLELKALK SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.
GALYGSPHFD LEDGYNVYQS ASRFLCQQPD AYPIPDSSPL YLYTDDDQDT TVVGAAHRSP ESLLELKALK PGVIQILGVK EAHLEIREDG QKDSPNQDAT EPLQGRSPSY SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV ASFD.
EAHLEIREDG LEDGYNVYQS ASRFLCQQPD AYPIPDSSPL YLYTDDDQDT TVVGAAHRSP ESLLELKALK PGVIQILGVK EAHGLPLRLP QKDSPNQDAT GALYGSPHFD PEACSFRELL SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY ASFD.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rMuFGF-21 contains less than 1 EU/μg. This information can be rearranged to say that the rMuFGF-21 has been found to possess a concentration lower than 1 EU/μg via the LAL method.

Activity

The standard and ED are comparable in terms of their complete biological activity. Please provide an alternate text generation method that produces highly similar content while ensuring that the generated content is distinct from the original text.₅₀The specific activity of less than 0.5 μg/ml, as determined by the thymidine uptake assay using BaF3 cells transfected with FGF-receptors, corresponds to a highly potent effect of > 2.0 × 10.³5 µg/mlrMuKlotho-β10 μg/ml，IU。

Reconstitution

It is recommended that the vial be given a brief spin in a centrifuge before opening, in order to settle the contents at the base. To reconstitute, use sterile distilled water or aqueous buffer with 0.1% BSA to create a concentration of 0.1-1.0 mg/ml. Stock solutions must be divided into operating aliquots and frozen at -20°C or below. Further dilutions can be made in the appropriate buffered solutions. To summarize, centrifugation, reconstitution in appropriate solutions, storage at low temperatures, and caution in dilution are all essential measures for handling this product.

Stability & Storage

This product, in its lyophilized state, can be stored at temperatures between 2-8 °C, but for long term preservation, it is recommended to store it at -20 °C, preferably in a desiccated environment. Once reconstituted, the product remains stable for up to 7 days at a range of temperatures between 2-8 °C. To guarantee maximum stability, the reconstituted product should be separated into smaller working aliquots and stored in a cold environment between -20 to -70 °C. It is essential to avoid multiple freeze/thaw cycles as this could affect the product's efficacy.

Target

Accession

Q9JJN1

Gene IDs

56636

References

References

1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.

2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.

3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95.