Recombinant Mouse FGF-18 #abs01011

abs01011-5ug

1-2 Weeks

138

abs01011-25ug

1-2 Weeks

344

abs01011-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, 500 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

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Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The content determined by the LAL method indicates that the rMuFGF-18 quantity is lower than 1 EU/μg.

Activity

Compared to the standard, it has been confirmed that the product is fully biologically active. The effective dose (ED) of this product is equivalent to that of the standard, indicating similarity in functionality.₅₀Our thymidine uptake assay, which utilized BaF3 cells transfected with FGF-receptors, yielded a result of less than 0.5 ng/ml. This indicates a high level of specificity, with a specific activity of over 2.0 × 10. We are confident in these findings and believe they are indicative of the potency of our FGF-receptors.⁶ IU/mg.

Reconstitution

To ensure optimal results, it is recommended to briefly centrifuge this vial before opening, so as to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA at a concentration of 0.1-1.0 mg/ml. It is advisable to divide the stock solution into aliquots and store them at -20°C or lower. Before use, dilute as required using suitable buffered solutions.

Stability & Storage

To ensure stability, it is recommended to store this lyophilized preparation at -20 °C with dry conditions for long term storage, although it can be kept at 2-8 °C as well. When reconstituted, the preparation remains stable for up to one week at 2-8 °C. For best results, divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. To maintain the integrity of the preparation, it is important to avoid subjecting it to repeated freeze/thaw cycles.

Target

Accession

O89101

Gene IDs

14172

References

References

1. Ohbayashi N, Hoshikawa M, Kimura S, et al. 1998. J Biol Chem. 273:18161-4.

2. Hu MC, Qiu WR, Wang YP, et al. 1998. Mol Cell Biol. 18:6063-74.

3. Itoh N, Ornitz DM. 2004. Trends Genet. 20:563-9.

4. Itoh N. 2007. Biol Pharm Bull. 30:1819-25.

5. Liu Z, Xu J, Colvin JS, et al. 2002. Genes Dev. 16:859-69.

6. Ohbayashi N, Shibayama M, Kurotaki Y, et al. 2002. Genes Dev. 16:870-9.

7. Usui H, Shibayama M, Ohbayashi N, et al. 2004. Biochem Biophys Res Commun. 322:887-92.

8. Ellsworth JL, Berry J, Bukowski T, et al. 2002. Osteoarthritis Cartilage. 10:308-20.

9. Moore EE, Bendele AM, Thompson DL, et al. 2005. Osteoarthritis Cartilage. 13:623-31.