Recombinant Mouse EGF #abs01015

abs01015-100ug

1-2 Weeks

138

abs01015-500ug

1-2 Weeks

344

abs01015-1mg

1-2 Weeks

393

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and lyophilized to create a powder. The powder was then reconstituted in PBS with a pH of 7.4.

Properties

aa_sequence

CGDYCVNSYG GCPSSY MNRWCLIDSG NWLSTVCIEG HDRLRWCRTD SGD ELR.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of rMuEGF, as detected using the LAL method, is below 1 EU/μg. Let me rephrase it using an alternative wording:
According to the results obtained from the LAL method, the measured amount of rMuEGF is less than 1 EU/μg.

Activity

The activity of the sample is just as potent as the standard on a biological level. The dosage necessary for an effective response is equivalent to that of the standard.₅₀Based on a cell proliferation assay using murine Balb/c 3T3 cells, it has been determined that the concentration of the substance is lower than 0.1 ng/ml. This indicates a high specific activity, exceeding 1.0 × 10.⁷ IU/mg.

Reconstitution

To ensure proper mixing, we suggest a brief centrifugation of the vial before opening. Resuspend the contents in sterile distilled water or an aqueous buffer that contains 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the stock solutions into working aliquots and store them at temperatures at or below -20°C. Subsequent dilutions should be made using appropriate buffered solutions.

Stability & Storage

For optimal storage conditions, it is recommended to keep this lyophilized preparation at a temperature range of 2-8 °C. However, for long term storage, it is advisable to store it at -20 °C while ensuring it is desiccated.
Once reconstituted, the preparation remains stable for a duration of one week when stored at 2-8 °C. To maintain maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures between -20 °C and -70 °C.
It is important to avoid exposing the preparation to repeated freeze/thaw cycles as it may compromise its stability.

Target

Accession

P01132

Gene IDs

13645

References

References

1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.

2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62.

3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58.

4. Cohen S. 2008. J Biol Chem, 283: 33793-7.