Recombinant Mouse VEGF120 #abs01016

abs01016-2ug

1-2 Weeks

138

abs01016-10ug

1-2 Weeks

344

abs01016-1mg

1-2 Weeks

5058

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The resulting powder was dissolved in PBS at a pH of 7.4. This process ensured that the solution was free from any contaminants that may have been present before filtration. The pH level of 7.4 was chosen to maintain the stability of the solution and prevent any degradation. Overall, this method of preparation ensured that the solution was of high quality and suitable for the intended application.

Properties

aa_sequence

CVPLMRCAGC YQRSYCRPIE TLVDIFQEYP RCECRPKKDR SHEVIKFMDV TSESNITMQI MAPTTEGEQK MRIKPHQSQH DEIEYIFKPS IGEMSFLQHS CNDEALECVP TKPEKCDKPR

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method showed that the rMuVEGF120 contained less than 1 EU/μg, indicating that the product has an extremely low level of endotoxin. This information can be rearranged to emphasize that the rMuVEGF120 product is of high quality and meets strict standards for endotoxin levels.

Activity

The standard and the ED were compared, and it was found that the ED is fully biologically active. It is important to generate highly similar content by rearranging the information from the original text.₅₀The specific activity of a substance, as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC), is greater than 2.0 × 10, with a corresponding concentration of less than 5 ng/ml. This information suggests that the substance is highly effective at stimulating cell growth and division in HUVEC, at very low concentrations. Further studies will be needed to investigate the potential applications of this substance in promoting angiogenesis, wound healing, or other biological processes that require cell proliferation.⁵ IU/mg.

Reconstitution

We suggest a brief centrifugation of the vial prior to opening to ensure that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer that contains 0.1% BSA and bring the concentration to 0.1-1.0 mg/mL. Divide the stock solutions into usable aliquots and store at temperatures lower than or equal to -20°C. Dilute further into the appropriate buffered solutions before use.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at a temperature range of 2-8°C. However, for long-term storage, it is best to keep it at -20°C and in a desiccated state. Once reconstituted, the preparation can be kept in the refrigerator for up to seven days. For optimal stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and freeze them at -20°C to -70°C. It is important to avoid repeated freeze/thaw cycles to prevent any degradation of the preparation. Follow these guidelines to ensure maximum performance and efficacy of the preparation.

Target

Accession

Q00731

References

References

1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.

2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.

3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.