Recombinant Human IGF-BP5 #abs00891

abs00891-5ug

1-2 Weeks

138

abs00891-25ug

1-2 Weeks

344

abs00891-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in 10 mM Sodium Citrate at pH 3.0 was filtered through a 0.2 μm filter and then lyophilized. This process resulted in a highly pure product that is now available for use. To ensure the best possible results, it is recommended to use this product according to the manufacturer's instructions. With its consistent quality and reliable performance, this lyophilized material is an excellent choice for a variety of research applications.

Properties

aa_sequence

ACEP DEKALSMCPP LGSFVHCEPC SPLGCELVKE PGCGCCMTCA TERCAQGLRC LAEGQSCGVY LGSFVHCEPC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE EPTTSEMAEE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA LGSFVHCEPC PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC SPLGCELVKE KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE LAEGQSCGVY.
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Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human IGF-BP5 contains less than 0.1 EU/μg as measured using the LAL method. It is important to note this level of endotoxin in the product to ensure its safety and efficacy.

Activity

The activity of the standard and the fully biologically active substances were compared, and it was found that they were closely similar. The effective dose of the biologically active substance was found to be equivalent to that of the standard.₅₀The specific activity of the substance, as measured by its ability to inhibit the proliferation of MCF-7 cells induced by IGF-II, is greater than 2500 IU/mg. This activity is observed at a concentration of less than 0.4 μg/ml in the presence of 15 ng/ml of Recombinant Human IGF-II.

Reconstitution

It is recommended to briefly centrifuge the vial before opening to ensure that the contents settle at the bottom. Add sterile distilled water or an aqueous buffer containing 0.1 % BSA to reconstitute at a concentration of 0.1-1.0 mg/mL. Store the stock solutions at ≤ -20 °C in working aliquots and make further dilutions in suitable buffered solutions. It is essential to follow these steps to ensure the quality and purity of the product.

Stability & Storage

To ensure the stability and longevity of this lyophilized preparation, it is recommended to store it at a temperature range of 2-8 °C. However, for long term storage, it is preferable to keep it at -20 °C in a desiccated condition. When reconstituted, the preparation remains stable for up to one week if stored at 2-8 °C. For optimal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures between -20 °C and -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may impact its viability.

Target

Accession

P24593

Gene IDs

3488