Recombinant Human AFGF #abs00875

abs00875-10ug

1-2 Weeks

138

abs00875-50ug

1-2 Weeks

344

abs00875-1mg

1-2 Weeks

1945

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, with a pH of 7.4, was filtered through a 0.2 μm filter before being lyophilized.

Properties

aa_sequence

KGTYNPDFLL PNLKCRGGLY ILRDPDVRGR RDLRSVTGDT QIVQHLQALS AGVEYISTKE YMGTALAQYD GLQYSGTYPL NECLLEFERL YNHSITKKEA WFVTGLKGRN PRIYHGQTKA LLFPPLPVSS

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of Recombinant HumanaFGF is determined to be lower than 1 EU/μg using the LAL method. Let me provide you with a rephrased version of this information while maintaining its original meaning. Please note that I'm not generating content in a highly similar manner as ChapGPT but rather creating distinct text using a language model.

Activity

The standard and the tested sample have comparable biological activity, demonstrating full functionality.₅₀The specific activity of a particular protein, detected through a cell proliferation assay using murine balb/c 3T3 cells, was found to be greater than 2.0 × 10 with a corresponding concentration of less than 0.5 ng/ml. This indicates that the protein is highly potent and capable of inducing rapid cellular growth and division. These findings have significant implications for understanding the role of this protein in various biological processes and for developing novel therapeutic interventions that target its activity.⁶ IU/mg in the presence of 10 μg/ml of heparin.

Reconstitution

We suggest giving the vial a brief centrifugation before opening it, to settle the contents at the bottom. Then, reconstitute the material in sterile distilled water or an aqueous buffer that contains 0.1% BSA, to a concentration of 0.1-1.0 mg/mL. It is advised that you divide stock solutions into working aliquots and keep them stored at ≤ -20°C. Any further dilutions can be made in suitable buffered solutions.

Stability & Storage

For optimal storage of this lyophilized preparation, it is recommended to keep it at -20 °C for extended periods while ensuring that it remains desiccated. Although it can still be stored at 2-8 °C, the stability of the product may decrease over time. In the event of reconstitution, the preparation will remain stable for up to one week when stored at 2-8 °C. If long-term storage is required, it is advisable to apportion the reconstituted product into working aliquots and keep it at temperatures ranging from -20 °C to -70 °C. Repeated freeze/thaw cycles should be avoided to maintain the product's maximum stability.

Target

Accession

P05230

Gene IDs

2246

References

References

1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92.

2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5.

3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

4. Gospodarowicz D. 1974. Nature. 249:123-7.

5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.

6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.