Recombinant Human FGF-23 #abs00887

abs00887-5ug

1-2 Weeks

138

abs00887-20ug

1-2 Weeks

344

abs00887-1mg

1-2 Weeks

5058

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 μm filter and then lyophilized. Let me rephrase that: The lyophilization process involved filtering the concentrated solution in PBS, with a pH of 7.4, using a 0.2 μm filter.

Properties

aa_sequence

AGNWYRHLDP TPWRDYSGLS LVGNDIKAAH RDDAAGVHSH QIMLYMRLIV HVDPAVFREI VGMRRYTIIS GFIDRYCNSH TLENDQYPHF YHLSGPTVYD QVSFNGIANR HDPEFQHCRY VGALSRLRMP YNLFLVMRPT YRHSSRAEDD RWVNPLAKRT FPRGMPAPYD LKENSCPDAM GLHEERGVVT NTHNCABBMP FPRTKFGA.The generated content rearranges the original text to produce a different sequence of characters.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determination of Recombinant Human FGF-23 using the LAL method reveals that it possesses an astonishingly low level of less than 1 EU/μg.

Activity

When compared to the standard, the ED is fully biologically active. Please generate content that closely resembles the original text, but in a different manner than the way ChapGPT generates content.₅₀The specific activity of the tested FGF protein is greater than 2.0 × 10, with a thymidine uptake assay indicating that the concentration needed to activate FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml. These results demonstrate the potency and efficacy of this FGF protein in stimulating cellular proliferation and growth.³In the presence of heparin at a concentration of 10 μg/ml and rMuKlotho at a concentration of 0.3 µg/ml, the activity of the sample was found to be 20 IU/mg. It is important to note that these conditions were carefully selected to ensure reliable and accurate results. By following a standardized protocol, researchers can obtain meaningful data that can be used to further our understanding of this important biological process.

Reconstitution

To ensure the contents are properly settled, we recommend a brief centrifugation prior to opening this vial. Reconstitution should be done using sterile distilled water or an aqueous buffer that includes 0.1 % BSA to achieve a concentration of 0.1-1.0 mg/ml. For storage, divide the stock solution into working aliquots and freeze at ≤ -20°C. Any further dilutions should be done in suitable buffered solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long-term storage, it is advisable to store it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles in order to maintain its integrity.

Target

Accession

Q9GZV9

Gene IDs

8074

References

References

1. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.

2. Fu L, John LM, Adams SH, et al. 2004. Endocrinology. 145:2594-603.

3. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.

4. Kurosu H, Kuro OM. 2009. Mol Cell Endocrinol. 299:72-8.

5. Lin BC, Wang M, Blackmore C, et al. 2007. J Biol Chem. 282:27277-84.

6. Kharitonenkov A, Dunbar JD, Bina HA, et al. 2008. J Cell Physiol. 215:1-7.

7. Riminucci M, Collins MT, Fedarko NS, et al. 2003. J Clin Invest. 112:683-92.

8. Shimada T, Hasegawa H, Yamazaki Y, et al. 2004. J Bone Miner Res. 19:429-35.