Recombinant Rat EGF #abs01077

abs01077-20ug

1-2 Weeks

138

abs01077-100ug

1-2 Weeks

344

abs01077-1mg

1-2 Weeks

1425

Species

Rat

Source

Escherichia coli.

Format

pH 7.4, lyophilized from a solution filtered through a 0.2 μm filter in PBS. Let's create a content that closely resembles the original information but presented differently.

Properties

aa_sequence

KLRRWWRCQH GYCLNGGVC DGYNSNTGCPPSY MYVESVDRYV CNCVIGYIGE

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rRtEGF contains less than 1 EU/μg. Let me create a rephrased version of the given information, keeping it consistent with the original text.

Activity

The standard and the ED are fully biologically active when compared.₅₀The specific activity of this substance, as measured by a cell proliferation assay utilizing murine Balb/c 3T3 cells, is greater than 1.0 × 10, with a concentration of less than 0.1 ng/ml. This information indicates that the substance has a potent cellular effect and is likely to be biologically active. It is important to continue exploring the potential applications of this substance, as it may have significant therapeutic potential.⁷ IU/mg.

Reconstitution

Prior to opening the vial, it is recommended to briefly centrifuge it in order to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer containing 0.1% BSA and aim for a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into smaller working aliquots and store them at temperatures at or below -20 °C. Any further dilutions should be made using appropriate buffered solutions.

Stability & Storage

This freeze-dried product can be stored for short-term use at 2-8 °C, but for long-term storage, it is recommended that it be kept at -20 °C, ideally in a desiccated state. After reconstitution, the product will remain stable for up to a week at 2-8 °C. To ensure the utmost stability, the reconstituted product should be divided into working aliquots and stored at -20 °C to -70 °C. It is important to avoid repetitive freeze/thaw cycles to maintain the product's quality and efficacy.

Target

Accession

P07522

Gene IDs

25313

References

References

1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.

2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62.

3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58.

4. Cohen S. 2008. J Biol Chem, 283: 33793-7.