Recombinant Rat VEGF164 #abs01078

abs01078-2ug

1-2 Weeks

138

abs01078-10ug

1-2 Weeks

344

abs01078-1mg

1-2 Weeks

5058

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8 was filtered through a 0.2 μm filter and then lyophilized. To elaborate, the original solution was subjected to a filtration process using a 0.2 μm filter to remove any impurities or particles. Subsequently, the resulting concentrated solution was subjected to lyophilization, which involves the removal of water by freeze-drying. The final product obtained after lyophilization is in the form of a solid powder.

Properties

aa_sequence

QEHKTGPMAT FVDMVKHEVA YRQCPIRYS ETPFLVDIYE FKYIEDEIPQ CRMCAGCVLP CEVLAENCDT QIMTQVNSSE HSQKQHPIRM LQFSMEGISH KDRKPCKRCE PCCHEKHRTK VFQLFHLRES CKCSCTQKDP RDNAKRCSTD RTECERRLNK RCKDPKRPSQ.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the determined level of rRtVEGF164 is below 1 EU/μg.

Activity

The standard and the compared sample are found to be fully biologically active. This implies that there are no significant differences in the biological activity between the two samples. The ED analysis confirms the similarity of both samples.₅₀According to the results of a cell proliferation assay using human umbilical vein endothelial cells (HUVEC), the concentration of the tested substance that induces cell growth is less than 5 ng/ml. This corresponds to a specific activity level of greater than 2.0 × 10, indicating a highly potent effect on the tested cells.⁵ IU/mg.

Reconstitution

To ensure proper contents, we suggest giving the vial a brief centrifugation before opening it, to bring the contents to the bottom. For reconstitution, use sterile distilled water or a buffer solution that contains 0.1% BSA, and aim for a concentration of 0.1-1.0mg/ml. Stock solutions should be divided into working aliquots and preserved at a temperature ≤ -20°C. Any additional dilutions should be made in an appropriate buffered solution. As a reminder, the generated content should not follow the format of ChapGPT-generated text, but rather be entirely unique.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should be stored at 2-8°C, but for long term storage, it is recommended to keep it at -20°C and desiccated. Upon reconstitution, the preparation can last up to one week at 2-8°C. For optimal stability, divide the reconstituted preparation into working aliquots and store them at -20°C to -70°C. It is important to avoid repeated freeze/thaw cycles as it can compromise the integrity of the preparation.

Target

Accession

P16612

Gene IDs

83785

References

References

1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.

2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.

3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.