Recombinant Rat HB-EGF #abs01079

abs01079-10ug

1-2 Weeks

138

abs01079-50ug

1-2 Weeks

344

abs01079-1mg

1-2 Weeks

1945

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, 300 mM NaCl, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. To preserve the stability of the lyophilized product, 5% trehalose was added. This process ensures that the resulting content is closely similar to the original text information.

Properties

aa_sequence

：
FVHHZSYZZZ SYUGRSNFPN EUROGKAANK DYPGKPGKGK GPCKLKGGKR YKYRGEILCD CIRYLLSPEP PHSCQHGCHY TPGQRHLGLC。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

LAL，rRtHB-EGF1 EU/μg。，。

Activity

The standard shows full biological activity when compared to the ED. Please produce a highly similar piece of information by reorganizing the provided content. The goal is to base the generated content on the original text, but in a different manner.₅₀According to a cell proliferation assay conducted using murine Balb/c 3T3 cells, the determined level of specific activity for the tested substance was greater than 1.0 × 10, with a corresponding concentration of less than 1 ng/ml. This indicates a potent biological effect of the substance on the cells.⁶ IU/mg.

Reconstitution

Before opening the vial, it is advisable to give it a brief centrifugation to settle the contents at the bottom. To prepare the protein for use, reconstitute it in sterile distilled water or an aqueous buffer that contains 0.1% BSA, until the desired concentration of 0.1-1.0 mg/mL is reached. The stock solutions ought to be separated into aliquots and stored at a temperature of less than or equal to -20 °C. When further dilutions are required, they should be mixed in suitable buffered solutions.

Stability & Storage

For long term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably desiccated. However, it can also remain stable at 2-8 °C. Upon reconstitution, the preparation can be stored for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller portions and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles. Please note that this response has been generated by rearranging the original content to provide a highly similar but distinct variation.

Target

Accession

Q06175

Gene IDs

25433

References

References

1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.

2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.