Recombinant Rat C10/CCL6 #abs01203

abs01203-2ug

1-2 Weeks

138

abs01203-10ug

1-2 Weeks

344

abs01203-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl was filtered through a 0.2 μm filter and subsequently lyophilized.

Properties

aa_sequence

PTISQIPRCS FTYFSTPYYI FVTIIPIKG CCFDSSDQGF HQIPTNPFRL KEDTVQDLGI CANPKRKRVR SGNSRLGKTP AIASSNQSQC.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of rRtC10/CCL6 in terms of endotoxin units per microgram is less than 1, as determined by the LAL method. Can you please produce a similar statement using different words, while retaining the information from the original text?

Activity

The biological activity of the standard can be fully matched by this compound. To determine its biological activity, a chemotaxis bioassay was conducted using murine BaF3 cells transfected with human CCR1. The concentration range required for this compound to exhibit its biological activity is between 10-100 ng/ml.

Reconstitution

Before opening, we advise briefly centrifuging the vial to ensure that the contents settle at the bottom. To reconstitute, mix with sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration between 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller working aliquots and store them at temperatures below -20 °C. Any further dilutions should be prepared using appropriate buffered solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long term storage, it is advisable to store it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for one week when stored at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may impact its stability.

Target

Accession

Q68FP3

Gene IDs

287910

References

References

1. Berger MS, Kozak CA, Gabriel A, et al. 1993. DNA Cell Biol. 12:839-47.

2. Orlofsky A, Berger MS, Prystowsky MB. 1991. Cell Regul. 2:403-12.

3. Ma B, Zhu Z, Homer RJ, et al. 2004. J Immunol. 172:1872-81.