Recombinant Human MIP-5/CCL15 #abs01134

abs01134-5ug

1-2 Weeks

138

abs01134-25ug

1-2 Weeks

344

abs01134-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20 mM PB, pH 7.4 and 100 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. A highly similar content can be created by rearranging the information in the original text, but using different wording.

Properties

aa_sequence

SYISQSIPC MMSKLPLENP VVLNSFHFAA DCCTQFINDAE KPYACMKKLV PGSKPGVIFL TKKGRQVCA ETSSECSFYP SLMLCCDTPG.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of Recombinant HumanMIP-5/CCL15 is determined to be less than 1 EU/μg using the LAL method. Can you come up with a similar statement by rephrasing the original text while preserving its meaning?

Activity

The standard is used as a benchmark to assess the biological activity of a substance, and it is found that the substance is fully biologically active when compared to the standard. In order to determine the biological activity, a chemotaxis bioassay is conducted using human T-lymphocytes. The results indicate that the substance exhibits significant biological activity within a concentration range of 1.0-10 ng/ml.

Reconstitution

It is recommended to give this vial a quick spin in the centrifuge before opening to ensure the contents settle at the bottom. Reconstitute with sterile distilled water or an aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions can be divided into working aliquots and stored at or below -20°C. Dilutions should be made in appropriate buffered solutions.

Stability & Storage

Storage of this lyophilized preparation is crucial for its stability and efficacy. It can be kept at 2-8 °C, but for long-term storage, it should be stored at -20 °C, preferably desiccated. Once reconstituted, it remains stable for up to one week at 2-8 °C. For maximum stability, the reconstituted preparation should be divided into working aliquots and stored at -20 °C to -70 °C. Avoiding repeated freeze/thaw cycles is also essential to maintain the integrity of the preparation. Overall, proper storage and handling of this lyophilized preparation are critical for ensuring optimal results.

Target

Accession

Q16663

Gene IDs

6359

References

References

1. Youn BS, Zhang SM, Broxmeyer HE, et al. 1998. Blood. 91:3118-26.

2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51.

3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.

4. Youn BS, Zhang SM, Lee EK, et al. 1997. J Immunol. 159:5201-5.

5. Coulin F, Power CA, Alouani S, et al. 1997. Eur J Biochem. 248:507-15.