Recombinant Human RANTES/CCL5 #abs01127

abs01127-5ug

1-2 Weeks

138

abs01127-20ug

1-2 Weeks

344

abs01127-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. To create a highly similar content, the information should be rearranged to state that the filtered concentrated solution was subsequently lyophilized.

Properties

aa_sequence

YINSLEMS RAHIKEYFYT CFAYIARPLP FVTRKNRQVC SGKCSNPAVV ANPEKKWVRE SPYSSDTTPC VVTKWPKWNC.
Rearrange the above content to generate a highly similar content while ensuring that the generated content is based on the original text information.
Please generate a highly similar content by rearranging the above content to form a new sequence. The new sequence should contain the same information as the original content. The rearranged sequence is:
YINSLEMS RAHIKEYFYT CFAYIARPLP FVTRKNRQVC SGKCSNPAVV ANPEKKWVRE SPYSSDTTPC.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the Recombinant Human RANTES/CCL5 contains less than 1 EU/µg. Please create a new piece of content that conveys the same information but is formulated differently and not based on the original text.

Activity

The standard and the fully biologically active form exhibit similar effectiveness. In order to determine the biological activity, a chemotaxis bioassay is conducted using human peripheral blood monocytes. The concentration range for this assay is between 1.0-10 ng/ml. It is important to note that the biological activity of the standard and the fully active form are comparable.

Reconstitution

Before opening the vial, we recommend briefly centrifuging it to ensure that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA to create a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at or below -20°C. Dilute further as needed in suitable buffered solutions. It's crucial to follow these steps to ensure proper handling and storage of the substance.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. For longer term storage, it should be kept at -20 °C, preferably desiccated. Upon reconstitution, the preparation can be used for up to one week at 2-8 °C. For optimal preservation, it is advised to divide the reconstituted preparation into working aliquots and store them at -20 °C. It is important to avoid repeated freeze/thaw cycles as it can potentially cause damage to the preparation.

Target

Accession

P13501

Gene IDs

6352

References

References

1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7.

2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34.

3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22.

4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8.

5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400.