Recombinant Human MCP-3/CCL7 #abs01128

abs01128-2ug

1-2 Weeks

138

abs01128-10ug

1-2 Weeks

344

abs01128-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm membrane before being lyophilized. The resulting product is a highly compacted powder that can be reconstituted in a suitable solvent to obtain a solution with the original concentration. This process ensures that the sample remains stable and viable for future use. The filtered solution is free of any contaminants, ensuring that the resulting lyophilized product is of the highest quality.

Properties

aa_sequence

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PKQRLESYRR QPVGINTSTT CCYRFINKKI TTSSHCPREA VIFKTKLDKE TQTPKL QDFMKHLDKK ICADPTQKWV

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human MCP-3/CCL7 is determined to be lower than 1 EU/μg as detected by the LAL method. Let me provide you with an altered version of the content based on the original text:
The LAL method analysis revealed that the Recombinant Human MCP-3/CCL7 has a concentration of less than 1 EU/μg.

Activity

In comparison to the standard, it has been verified that the product is completely biologically active. The bioactivity was assessed utilizing human monocytes in a chemotaxis bioassay and confirmed to be within a range of 10-100 ng/ml.

Reconstitution

Prior to opening, it is recommended to briefly centrifuge the vial in order to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA and aim for a concentration of 0.1-1.0 mg/mL. Divide the stock solution into working aliquots and store them at or below -20 °C. When making further dilutions, use suitable buffered solutions.

Stability & Storage

For optimal storage of this lyophilized preparation, it is recommended to keep it at a temperature range of 2-8 °C. However, for long term storage, it is preferable to store it at -20 °C, making sure it is desiccated to maintain its stability. Once reconstituted, the preparation remains stable for one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at -20 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to maintain its integrity.

Target

Accession

P80098

Gene IDs

6354

References

References

1. Opdenakker G, Fiten P, Nys G, et al. 1994. Genomics. 21:403-8.

2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.