Recombinant Human Eotaxin/CCL11 #abs01130

abs01130-5ug

1-2 Weeks

138

abs01130-20ug

1-2 Weeks

344

abs01130-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. A highly similar content can be generated by rearranging this information to read: After undergoing filtration with a 0.2 μm filter, the concentrated solution in 20 mM PB at pH 7.4 with 150 mM NaCl was subjected to lyophilization.

Properties

aa_sequence

PKPSTKQPSD YLKMDQSPKK DQAWVKKKPD ICDAKLKTKF IVAKQPCGKS TIRRYSELRQ LPINRANFLC CPTVSAVGPF RITTYYYRSE KILNRNALFN.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the Recombinant Human Eotaxin/CCL11 contains less than 1 EU/μg. It is important to note that this content is highly similar to the original information provided.

Activity

The standard and the fully biologically active counterpart exhibit comparable activity. To assess the biological activity, a chemotaxis bioassay was conducted using human peripheral blood eosinophils. The concentration range for determining the activity was found to be between 0.1-10.0 ng/ml. By rearranging the information provided, it can be stated that the biological activity of the fully active version closely resembles that of the standard.

Reconstitution

We suggest that the vial be given a short centrifugation before opening to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer which includes 0.1% BSA to get a concentration of 0.1-1.0 mg/mL. Prepare stock solutions by dividing them into working aliquots and keep at ≤ -20 °C. To make further dilutions, use the appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C, but it is recommended to keep it at -20 °C for long-term storage. It is preferable to store it in a desiccated condition. Once reconstituted, the preparation remains stable for one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P51671

Gene IDs

6356

References

References

1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12.

2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7.

3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56.

4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.