Recombinant Human MCP-4/CCL13 #abs01131

abs01131-5ug

1-2 Weeks

138

abs01131-20ug

1-2 Weeks

344

abs01131-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

Rearranged content: The concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

The sequence QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT can be rearranged to form a new sequence of characters that retains the same information. Here is one possible reordering:
TLKTHLGRKAMNYKPVEWKKDPCAIEKGKLTRFIVAKQPCRTVSYYSLFSCFNTDQ
This new sequence contains the same set of characters as the original, but in a jumbled order. It is up to the reader to interpret this new sequence and derive meaning from it, as the original text was not particularly meaningful in and of itself.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that Recombinant Human MCP-4/CCL13 has a concentration of less than 1 EU/μg. This data indicates its low endotoxin level.

Activity

The standard and the analyzed substance exhibit complete biological activity, with no significant difference observed. The level of biological activity was evaluated through a chemotaxis bioassay that utilizes human monocytes. The concentration range of the substance that displayed the desired biological response was between 10-100 ng/ml.

Reconstitution

It is recommended to briefly centrifuge the vial before opening to ensure the contents settle at the bottom. To reconstitute, add sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a 0.1-1.0 mg/mL concentration. Stock solutions should be divided into working aliquots and stored at ≤ -20°C, while further dilutions can be made in appropriate buffered solutions. It's essential to follow these guidelines for optimal results.

Stability & Storage

To keep this lyophilized preparation intact, it must be stored between 2-8 °C, and for prolonged storage, it is recommended to keep it at -20 °C while ensuring it is desiccated. Once reconstituted, the preparation can last for up to a week at 2-8 °C. For utmost stability, divide the reconstituted preparation into working aliquots and keep it between -20 °C and -70 °C. Avoid repeating the freeze/thaw cycles as it may affect the stability of the preparation.

Target

Accession

Q99616

Gene IDs

6357

References

References

1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84.

2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13.

3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26.