Recombinant Rat MIP-1β/CCL4 #abs01202

abs01202-5ug

1-2 Weeks

138

abs01202-20ug

1-2 Weeks

344

abs01202-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH 7.4, 3% trehalose was filtered through a 0.2 μm filter and then subjected to lyophilization.

Properties

aa_sequence

VFLTKKGRQI CCFSYTSRKI CADPSEPWVN APIGSDPPTS TSSLCSQPAV HRNFVMDYYE EYVNDLELN. It is important to use the original information from the above content to create a highly similar content by rearranging the sequence of the words. By doing so, we can ensure that the generated content is based on the original text information.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method indicates that the rRtMIP-1β/CCL4 sample contains less than 1 EU/μg. This information can be rearranged to produce a similar content that accurately conveys the original message.

Activity

The standard and fully biologically active compound can be compared. Its biological activity is assessed through a chemotaxis bioassay that utilizes human peripheral blood monocytes. The concentration range for this activity is 10-1000 ng/ml.

Reconstitution

Prior to opening the vial, we recommend a brief centrifugation to ensure that the contents are at the bottom. Reconstitution can be achieved by using sterile distilled water or an aqueous buffer containing 0.1% BSA, at a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at temperatures equal to or below -20°C. For further dilutions, it is recommended that appropriate buffered solutions are used.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it is best to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. For optimal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P50230

Gene IDs

116637

References

References

1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.

2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.

3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.