Recombinant Mouse KC/CXCL1 #abs01154

abs01154-5ug

1-2 Weeks

138

abs01154-20ug

1-2 Weeks

344

abs01154-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was subjected to filtration with a 0.2 μm filter and subsequently lyophilized to obtain a dry powder. The resulting content is highly similar to the original text information, but rearranged for better clarity and understanding.

Properties

aa_sequence

LPGHITQNCA KLQCIHMAGE VNPSLKTQIL QECTHPTGVS GEARLKNITD VLQADPKPEC GVKGMLKKVI QP.
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The sequence of letters in the text is rearranged in the above content, but it still conveys the same information as the original text. It seems to be a randomly arranged set of letters, but it may have some hidden meaning. Perhaps it is a code or a message waiting to be deciphered.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

As determined by the LAL method, the level of rMuKC/CXCL1 is less than 1 EU/μg. Please generate a similar text by rearranging the original content, while ensuring that the newly-generated text accurately represents the information in the original text.

Activity

The standard and the fully biologically active form exhibit comparable levels of biological activity. To determine this activity, a chemotaxis bioassay is conducted using human peripheral blood neutrophils. The concentration range required for this assay is between 10-100 ng/ml.

Reconstitution

Before opening this vial, we suggest briefly centrifuging it to ensure the contents settle at the bottom. To reconstitute, add either sterile distilled water or an aqueous buffer with 0.1% BSA to obtain a concentration of 0.1-1.0 mg/mL. It's important to divide solutions into working aliquots and store them at or below -20 °C. For further dilutions, use the appropriate buffered solutions.

Stability & Storage

To maintain the viability of this lyophilized preparation, it is recommended to store it at a temperature ranging from 2-8 °C. However, for longer shelf life, it should be kept at a lower temperature of -20 °C, with a preference towards desiccation. After reconstitution, the product remains stable for up to a week if stored in the 2-8 °C temperature range. To ensure the preparation's optimal stability, it is advisable to divide and store the reconstituted product into working aliquots, which should be kept at -20 °C to -70 °C. Always avoid repetitive cycles of freeze-thaw, as it can compromise the preparation's quality.

Target

Accession

P12850

Gene IDs

14825

References

References

1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.

2. Anisowicz A, Bardwell L, Sager R. 1987. Proc Natl Acad Sci U S A. 84:7188-92.

3. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98.

4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.

5. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

6. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802.

7. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44.