Recombinant Human CD47/IAP/OA3 (C-6His) #abs04007

abs04007-10ug

1-2 Weeks

123

abs04007-50ug

1-2 Weeks

337

abs04007-500ug

1-2 Weeks

2353

abs04007-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system is utilized to produce Recombinant Human CD47. The target gene, which encodes Gln19-Pro139, is expressed with a 6His tag at the C-terminus. To create a similar content, we can rearrange the information as follows: The production of Recombinant Human CD47 is carried out using our Mammalian expression system. This system allows for the expression of the target gene, which encodes Gln19-Pro139, with a 6His tag at the C-terminus.

Other names

The Leukocyte Surface Antigen CD47 is also known as Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, IAP, Protein MER6, or simply MER6. This protein is involved in a variety of biological processes such as cell migration, phagocytosis, and immune response. CD47 is located on the surface of many different types of cells including leukocytes, red blood cells, and platelets. It functions as a "don't eat me" signal to prevent phagocytosis by macrophages and other immune cells. In addition, CD47 can interact with a variety of other proteins and may play a role in cell signaling and adhesion. Understanding the function of CD47 is important for developing new therapies for cancer, autoimmune diseases, and other disorders.

Source

Human Cells

Format

The contents were freeze-dried after being passed through a 0.2 μm filter. The solution consisted of 10mM Tris-Citrate, 150mM NaCl, and had a pH of 8.0. A highly similar content can be produced by reshuffling the original text as follows: The solution of 10mM Tris-Citrate and 150mM NaCl, with a pH of 8.0, was filtered through a 0.2 μm filter before being freeze-dried.

Properties

aa_sequence

FTCFVKNDPTVEKGRDNSVIAQTVTKSLEKYTFDWSSQEVNKKVMKFALTDNSTYPFDGAIYAKTTKFEQWVRENTVLHHHHTDFVPSAKIACLSGEVMDLKSDAVTWFKGTCEEREYTVNTQNNALFEVK.Please create a highly comparable statement through the repositioning of the initial text provided above. The proposed statement should be closely aligned with the original text's information but should differ significantly in its presentation.

Concentration

The determination of exceeding 95% was made by employing SDS-PAGE gel electrophoresis method. Let me provide you with a rearranged version of the given content based on the provided information.
The evaluation of surpassing 95% was accomplished by implementing SDS-PAGE gel electrophoresis, a technique widely used in biochemistry and molecular biology.

Endotoxin_level

The LAL test has shown that the concentration of endotoxin present is less than 0.1 ng/μg, which is equivalent to 1 IEU/μg. This indicates that the sample contains an extremely low level of endotoxin.

Activity

The ability to bind Human SIRPA in functional ELISA was measured. The effect's ED50 is below 50 ng/ml. A content with similar meaning can be created by rearranging the information from the original text, while ensuring that the generated content is linguistically distinct from the ChapGPT-generated responses.

Reconstitution

To ensure optimal results, it's important to always centrifuge tubes before opening and avoid mixing by vortex or pipetting. For best results, it is recommended that the lyophilized protein is dissolved in ddH2O and reconstituted to a concentration no less than 100 μg/ml. To minimize freeze-thaw cycles, be sure to aliquot the reconstituted solution. It's worth noting that it's not advisable to reconstitute the protein to a concentration lower than 100 μg/ml. Follow these guidelines closely to achieve the best possible outcomes.

Stability & Storage

The recommended storage conditions for lyophilized protein are below -20°C. However, it is worth mentioning that the protein remains stable at room temperature for a period of 3 weeks. On the other hand, once the protein is reconstituted and in solution form, it can be stored at temperatures ranging from 4°C to 7°C for a duration of 2 to 7 days. It is also important to note that if you prefer to store aliquots of the reconstituted samples, they can be kept below -20°C for up to 3 months, maintaining their stability.

Target

Background

CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Accession

Q08722