Recombinant Mouse BD-2 #abs01034

abs01034-5ug

1-2 Weeks

138

abs01034-20ug

1-2 Weeks

344

abs01034-1mg

1-2 Weeks

4214

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to create a dry powder. This process ensures the preservation of the sample while also making it easier to handle and store. The resulting product can be easily reconstituted by adding water or other appropriate solvents. The use of lyophilization is a common technique in many fields, including medicine, biotechnology, and food science, as it allows for long-term storage of sensitive samples without compromising their quality.

Properties

aa_sequence

PPSKSRCFPA CVRAINEYSSG APCYCMHHID KENSEAKVCI GLGTYGPRAS GMPKGCHLEY

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rMuBD-2 has a level of less than 1 EU/μg. This information can be used to establish highly similar content by rearranging the original text.

Activity

In a concentration range of 10-100 ng/ml, the biologically active level of a substance is found to be fully comparable to the standard. This determination is made through a chemotaxis bioassay, which involves using immature human dendritic cells. By assessing their response to the substance, its biological activity is confirmed.

Reconstitution

It is advisable to briefly centrifuge this vial before opening it to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer that contains 0.1 % BSA to achieve a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into separate working aliquots and store them at a temperature of not more than -20 °C. If there is a need for further dilution, it should be carried out in appropriate buffer solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at a temperature range of 2-8 °C. However, for long-term storage, it should be stored at -20 °C, preferably in a desiccated state. After reconstitution, the preparation can be stored for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is advisable to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P82020

Gene IDs

13215

References

References

1. Rivas-Santiago B, Cervantes-Villagrana A, Sada E, et al. 2012. Arch Med Res, 43: 324-8.

2. Poiraud C, Quereux G, Knol AC, et al. 2012. Eur J Dermatol, 22: 634-9.

3. Mullin J, Carter S, Williams N, et al. 2012. Vet Microbiol.