Rat IL-1β High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs553801-96T	4 weeks	$580.00

Please note that the price provided above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.

Testing Principle：

The double antibody sandwich ELISA kit employs a specific anti-human CXCL13 Capture Antibody that is already coated on a high affinity conjugate plate. The procedure begins by sequentially adding a standard substance, the sample to be tested, and a biotin-labeled detection antibody into the well of an HRP plate. The mixture is thoroughly mixed, allowing for a 2-hour incubation of the CXCL13 in the sample at room temperature. During this incubation process, the CXCL13 in the sample combines with the solid-phase antibody and the detection antibody. To eliminate any unbound components, the well is thoroughly washed, and horseradish peroxidase-labeled streptavidin-HRP (SA-HRP) is added. After washing again to remove excess SA-HRP, TMB color substrate is introduced and incubated in darkness at room temperature to initiate color development. The intensity of the color reaction is directly proportional to the concentration of CXCL13 in the sample. To stop the reaction, a termination solution is added, and the absorbance value is measured using a microplate analyzer at a detection wavelength of 450 nm (with a correction wavelength range of 570-630 nm).

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The 96-well plate is pre-coated with all the necessary components to perform a CXCL13 test, including Standards, a test antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, and SA-HRP. In addition, the kit comes with a Seal Plate Film and Instructions to ensure that the testing process is streamlined and accurate. With these materials, users can easily and effectively detect the presence of CXCL13 in their samples, enabling them to make informed decisions based on their research needs.

Sensitivity:0.71 pg/mL

Detection range： 7.81-500 pg/mL

Recovery rate: 89-127%

Storage：2-8℃

The standard curve：

 

Background：

Interleukin 1 (IL-1) is a peptide of 17kDa that is present extracellularly and divided into two proteins, IL-1α and IL-1β. The latter is secreted by activated macrophages and is produced through the process of hydrolysis of caspase1 (CASP1/ICE). This cytokine is a crucial mediator of the inflammatory response and plays a crucial role in many cellular processes such as cell proliferation, differentiation, and apoptosis. The family of IL-1 proteins is known to originate from biologically active monocytes and is heavily involved in immune and inflammatory responses. When IL-1β is expressed at high levels, it can lead to numerous autoinflammatory syndromes, with CAPS being notably prevalent. CAPS, which is caused by inflammasome receptor NLRP3 mutations that trigger IL-1β processing, falls under the category of single-gene conditions that can arise due to elevated IL-1β expression.

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