Human IL-9 ELISA Kit

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Testing Principle：

The double antibody sandwich ELISA is employed in this kit to detect human IL-9. The high affinity conjugate plate is pre-coated with specific anti-human IL-9 capture antibody. The standard substance, as well as the sample and detection antibody labeled with biotin are sequentially added into the HRP-plate well. After thorough shaking and mixing, the IL-9 present in the sample undergoes a 2-hour incubation process at room temperature, during which it binds to the solid phase antibody and the detection antibody. Subsequently, free and unbound ingredients are removed by extensive washing, following which horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. The plate is washed again and TMB color substrate is added, which is then allowed to develop color in the dark, at room temperature. The intensity of the color reaction is positively proportional to the concentration of IL-9 in the sample. The reaction is stopped by adding the termination solution and the absorbance value is measured at 450 nm detection wavelength, with correction wavelength 570-630 nm, using a microplate analyzer.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The kit includes a pre-coated 96-well plate, Standard, IL-9 test antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Mask, and Instructions. These components are essential for conducting an IL-9 test.
To begin the assay, the pre-coated 96-well plate is provided, which acts as the solid phase support. The plate is already coated with the necessary reagents for capturing IL-9.
A Standard is included in the kit for establishing a calibration curve. This Standard consists of known concentrations of IL-9, allowing for quantitative analysis.
The IL-9 test antibody is also part of the kit, specifically designed to bind to IL-9. It enables the detection and measurement of IL-9 in the test samples.
Standard Diluent is provided to dilute the Standard and other samples, ensuring that they are within the detection range of the assay.
Test Buffer is included to create the desired reaction environment for the assay. It provides the necessary conditions for the interaction between the IL-9 test antibody and IL-9 in the samples.
TMB Color Substrate is included as the enzymatic substrate. It produces a colored reaction product when acted upon by the enzyme.
Washing Solution is vital for cleaning the wells and removing any unbound substances during the assay. It ensures accurate and reliable results.
Stop Solution is provided to terminate the enzyme-substrate reaction, preventing any further color development.
SA-HRP, an enzyme conjugate, is included to facilitate the detection of IL-9. It binds to the IL-9 test antibody and initiates the enzymatic reaction.
A Seal Plate Mask is also included to cover and protect the plate during incubation and shaking steps, minimizing evaporation.
Lastly, the kit includes detailed Instructions to guide the user through the entire IL-9 testing procedure. These instructions explain the steps involved, precise measurements, and the interpretation of results.
Overall, this comprehensive kit provides all the necessary components for performing an IL-9 test efficiently and accurately.

Sensitivity：0.06 pg/mL

Detection range： 7.81 -500 pg/mL

Recovery rate： 80-117%

Storage: 2-8℃

The standard curve：

Background：

IL-9, also known as Interleukin 9, is a glycosylated protein weighing 14kDa. It possesses 10 cysteine residues that play a role in disulfide bonding. T cells, particularly CD4+ helper cells, are responsible for producing this cytokine, which regulates various hematopoietic cells. Its primary functions include promoting cell proliferation and preventing cell apoptosis. IL-9 exerts its effects by binding to the IL-9 receptor and activating different signal transduction and activation (STAT) proteins. Consequently, it participates in multiple biological processes. Studies have implicated the gene encoding IL-9 as a potential candidate gene for asthma. In mouse models, IL-9 has been deemed a critical factor in the development of bronchial hyperresponsiveness, which is a characteristic of asthma. Furthermore, the presence of an IL-9-mediated autocrine loop suggests its involvement in certain malignancies like Hodgkin's disease. Interestingly, IL-9 is expressed in Lee-Scherer cells, Hodgkin's lymphoma cells, and some large aplastic lymphoma cells. However, it is not present in non-Hodgkin's lymphoma and peripheral T-cell lymphoma.

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