Human IL-1α ELISA Kit

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Testing Principle：The double antibody sandwich ELISA technique is utilized in this kit to detect human IL-1α. Specifically, a capture antibody designed to target human IL-1α is already placed onto a conjugate plate. Sample standards, as well as test samples and detection antibodies labeled with biotin, are added sequentially into the HRP-plate wells. The IL-1α present in the samples gets bound to the solid-phase and detection antibody after a two-hour incubation process at room temperature. Following thorough washing to get rid of any free or unbound components, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. After another round of washing, TMB color substrate is added, and the resulting mixture is incubated in the dark to allow color development with the depth of the color equal to the concentration of IL-1α in the sample. The termination solution halts the reaction, and the absorbance value is recorded at a detection wavelength of 450 nm (with correction wavelength 570-630 nm) using a microplate analyzer. 

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The 96-well plate is pre-coated and comes with a sealing plate membrane. It includes standards for measuring IL-1α antibodies. To dilute the standards, you can use the standard diluent. For the test samples, a specific buffer is provided. To detect the IL-1α antibodies, an SA-HRP solution is used. The TMB color substrate is employed for the development of color in the assay. Additionally, there is a sample activation fluid provided to activate the samples before analysis. For cleaning the plate, a scrubbing solution is available. Finally, the reaction can be stopped using the provided stop buffer.

Sensitivity：0.52 pg/mL

Detection range： 7.81-500 pg/mL

Recovery rate： 81-106%

Storage：2-8℃

The standard curve：

Background：

IL-1 is a peptide that weighs 17kDa and is found outside the cells. It is composed of two proteins called IL-1α and IL-1β. In humans, IL-1α is encoded by a gene called IL1A. This gene is responsible for producing IL-1α, which is mainly released by activated macrophages, neutrophils, epithelial cells, and endothelial cells. IL-1α has various functions including metabolic, physiological, and hematopoietic activities. Its primary role is to regulate the immune response, making it a crucial player in the body's defense system.
HAX1 and NDN are two proteins that can interact with IL-1α, though their clinical relevance is not as significant as the synergistic effect between IL-1α and TNF. These two cytokines, IL-1α and TNF, are both classified as acute phase cytokines, playing a crucial role in inducing fever and inflammation in the body.

IL-1α is currently being evaluated as a potential tumor marker in clinical trials.  Blocking IL-1α activity has potential for treating skin diseases, such as acne.  

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