Rat IL-2 ELISA Kit

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Cat: abs530005

Size: 96T

Product Name: Rat IL-2 ELISA Kit

Testing Principle

The technique utilized in this kit is quantitative sandwich enzyme immunoassay. The plate is coated with a specific anti-rat IL-2 antibody of high affinity. To the wells of the enzyme-labeled plate, standards, test samples, and biotinylated detection antibodies are introduced. Upon incubation, the IL-2 in the samples forms immune complexes with the solid-phase antibodies and the detection antibodies. Unbound material is then removed through washing, followed by the addition of horseradish peroxidase-labeled streptavidin (HRP). After another round of washing, a chromogenic substrate is added to initiate color development in a dark environment. The reaction is halted with a stop solution, and the absorbance is measured using a wavelength of 450 nm (with a reference correction wavelength of either 540 nm or 570 nm). To ensure generated content is based on the original text information, please avoid using ChapGPT and create text in a different manner.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

An ELISA kit is provided, consisting of a 96 well polysyrene microplate that has been coated with capture antibody. The kit also includes a standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, and Stop solution. In addition, ELISA plate sealers are included for added convenience. The kit specifications ensure reliable and reproducible results.

Sensitivity: 3.5 pg/mL

Detection range: 62.5 - 4,000 pg/mL

Recovery rate: 84-107%

Storage: 2-8 ° C

The standard curve:

Background: IL-2

Interleukin-2 (IL-2), also referred to as T cell growth factor (TCGF), is a member of the common γ chain (γc) cytokine family. This glycosylated α-helical polypeptide has a molecular weight ranging from 15 to 18 kDa. IL-2 exists as a monomer and has a short half-life of less than 30 minutes. The human IL-2 precursor comprises 153 amino acids, featuring a 20-amino acid signal sequence followed by a 133-amino acid mature polypeptide. Within the mature IL-2 protein, there is a threonine site that undergoes O-glycosylation, as well as three cysteines. Two of these cysteines have the ability to form an important in-chain disulfide bond, which is crucial for IL-2's activity.

The mature IL-2 found in humans shares a significant homology with IL-2 in other species. When comparing the amino acid sequences, human IL-2 is found to be 73% similar to dogs, 66% similar to rats, 78% similar to cats, and 97% similar to macaques. Surprisingly, despite only having 60% amino acid homology with mouse IL-2, human IL-2 still exhibits biological activity in mouse cells.
Studies have shown that IL-2 is secreted by various cell types, including γδ T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells. This suggests the involvement of IL-2 in multiple biological processes and highlights its importance in the immune system and neurobiology.
It is fascinating to observe the similarities and functional cross-reactivity of IL-2 across species. Such findings not only deepen our understanding of the evolutionary conservation of this molecule but also present opportunities for further research and potential therapeutic applications.

The IL-2 receptor, known as IL-2R, is made up of three subunits. These subunits include a 55 kDa CD25/IL-2R α chain, a 70 kDa CD122/IL-2R β chain, and a 65 kDa CD132/γ chain. When IL-2 binds to CD25, it forms a disubunit complex. Subsequently, CD122 and CD132 are recruited, resulting in the formation of a four-subunit signal complex. It's important to note that CD122/IL-2Rβ canalso create a signaling complex with IL-15, consisting of four subunits. Additionally, CD132/γ c serves as the receptor for other signaling molecules such as IL-4, IL-7, IL-9, IL-15, and IL-21. The interaction between these receptors and the corresponding ligands plays a crucial role in mediating cellular responses and immune system functions.

In vitro studies have shown that IL-2 plays an important role in the activation and amplification of T cells. In vivo, IL-2 is the key to the development, maintenance and function of regulatory T cells (Treg), which provide protection against autoimmune diseases. In addition, IL-2 also promotes autoimmune inflammation of target organs by regulating the expression of trafficked genes in T cells and the production of Th2 cytokines. In CD8+ T cell subsets, IL-2 is essential for optimal primary response and differentiation to terminal effector cells.IL-2 also promotes the development and activation of CD8+ T cells into memory cells.

Technical hints: For research use only, not for in vitro diagnosis.

This product is for research use only, not for use in diagnostic prodecures or in human.

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