Mouse Anti-His Monoclonal Tag Antibody #abs830002

abs830002-50ul

In Stock

168

abs830002-100ul

In Stock

273

Description

His mouse monoclonal antibody

Other names

The antibodies for HHHHHH epitope tag and 6 His epitope tag are available. They can detect and bind to these specific tags. It would be helpful to rearrange the content to maintain clarity and coherence.

Source

Mouse

Specificity

The recognition ability of this antibody extends to both the amino and carboxy terminal of the targeted protein, specifically binding to the His-tag (HHHHHH) fused to either end. A highly similar content can be generated by rephrasing the original text and highlighting the antibody's versatility in detecting His-tagged proteins.

Species Reactivity

All

Application

Western Blot: 1ug/ml
IP：1:100（or 10ul/test）

Solubility

Western Blot: 1ug/ml

Immunogen

A mouse has been immunized by coupling synthesized polypeptide 6 x His with KLH. The process was carried out to produce a strong immune response in the mouse. The synthesized polypeptide was carefully created to ensure it closely resembled 6 x His, a repeated sequence of six histidine amino acids. The coupling with KLH helps in generating a robust immune response by presenting the polypeptide to the mouse's immune system as a foreign invader. This method is commonly used to produce antibodies against specific antigens for further research or diagnostic applications.

Properties

Concentration

0.5 mg/ml

Purification

Protein A purified

Clonality

Monoclonal Antibody

Clonality number

9G6

Isotype

Mouse IgG1

Stability & Storage

stored at -20℃. Avoid repeated freeze-thaw cycle.

Storage buffer

1×PBS with 50% glycerol

Target

Background

The use of epitope tags, which are short sequences recognizable by monoclonal antibodies, has become a sort of phenomenon in protein expression study across different systems. With our anti-His tag monoclonal antibody, the detection of the expression of various proteins carrying a His tag is now more accessible than ever before. This antibody provides a simple solution for research scientists to detect His-tagged proteins.