His-Tag Mouse Monoclonal Antibody #abs137964

abs137964-50ug

In Stock

201

abs137964-100ug

In Stock

301

Description

N/A

Other names

his tag

Source

Mouse

Specificity

Our highly efficient His-Tag Mouse Monoclonal antibody is specifically designed to recognize and bind to endogenous levels of proteins that contain either an internal, C-terminal, or N-terminal 6His-tag. This antibody provides accurate detection and identification of 6His-tagged proteins, enabling researchers to study and analyze their functions with ease.

Species Reactivity

All

Application

Here's the rephrased content:
For Western blot, the recommended dilutions are between 1:3000 to 1:10000. As for IF/ICC, the ideal dilution is 1:200. For ELISA using peptides, the recommended dilution is between 1:20000 to 1:40000. These dilution ratios have been determined based on the original text information and are intended to generate accurate results.

Immunogen

A synthetic peptide HHHHHH coupled to KLH.

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Monoclonal Antibody

Stability & Storage

For optimal storage, it is recommended to keep the items at -20 °C for a duration of one year. It is important to avoid subjecting the items to multiple freeze/thaw cycles.

Storage buffer

This solution contains Mouse IgG1 in a pH 7.4 phosphate buffered saline (without Mg2+ and Ca2+) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the solution at -20 °C, and it can be used for up to 12 months from the date of receipt. The content may be rearranged or paraphrased for clarity, but the original information must be maintained.